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PXD034650

PXD034650 is an original dataset announced via ProteomeXchange.

Dataset Summary
Title: Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair
DescriptionBRD4 functions as an epigenetic reader and plays a crucial role in regulating transcription and genome stability. Dysregulation of BRD4 is frequently observed in various human cancers. However, the molecular details of BRD4 regulation remain largely unknown. Here, we report that PRMT2- and PRMT4-mediated arginine methylation is pivotal for BRD4-dependent transcription, DNA repair, and tumor growth. Specifically, PRMT2/4 interact with and methylates BRD4 at R179, R181, and R183. This arginine methylation selectively controls a transcriptional program by promoting BRD4 enrichment at the hyper-acetylated chromatin regions. Moreover, BRD4 arginine methylation is induced by DNA damage and thereby promotes its binding to chromatin for DNA repair. Deficiency in BRD4 arginine methylation significantly suppresses tumor growth and sensitizes cells to BET inhibitors and DNA damaging agents. Therefore, our findings reveal an arginine methylation-dependent regulatory mechanism of BRD4 function and highlight targeting PRMT2/4 for better anti-tumor effect of BET inhibitors and DNA damaging agents.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:07:26.986.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJennifer Bethard
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonomethylated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-19 05:10:35ID requested
12023-03-10 19:26:31announced
22023-11-14 07:07:27announced2023-11-14: Updated project metadata.
Publication List
Liu L, Lin B, Yin S, Ball LE, Delaney JR, Long DT, Gan W, Arginine methylation of BRD4 by PRMT2/4 governs transcription and DNA repair. Sci Adv, 8(49):eadd8928(2022) [pubmed]
Keyword List
submitter keyword: transcription, DNA repair, arginine methylation, PRMT2/4,BRD4
Contact List
Wenjian Gan, Ph.D.
contact affiliationDepartment of Biochemistry and Molecular Biology Medical University of South Carolina
contact emailganw@musc.edu
lab head
Jennifer Bethard
contact affiliationMedical University of SC
contact emailbethard@musc.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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