PXD034649

PXD034649 is an original dataset announced via ProteomeXchange.

Title	Eps8, Arp2/3 and IRSp53 in TNT formation
Description	Tunneling nanotubes (TNTs) connect distant cells and mediate cargo transfer for intercellular communication in physiological and pathological contexts. How the cell controls a common pool of proteins to generate these protrusions spanning length scales beyond those attainable by canonical filopodia remains unknown. Through a combination of surface micropatterning and optical tweezer-based approaches, we found that Arp2/3-dependent pathways attenuate the extent with which actin polymerizes in nanotubes, limiting the formation and attainable lengths of TNTs. Proteomic analysis using Epidermal growth factor receptor kinase substrate 8 (Eps8) as a positive effector of TNTs showed that upon Arp2/3 inhibition, proteins enhancing filament turnover and depolymerization were reduced and instead Eps8 exhibited heightened interactions with the inverted Bin/Amphiphysin/Rvs (I-BAR) domain protein IRSp53 that provides a direct connection with linear actin polymerases. Our data reveals how common players in protrusions (Eps8 and IRSp53) facilitate the formation of TNTs, and that this Eps-IRSp53 interaction is enhanced when competing pathways overutilizing actin in branched Arp2/3 networks are inhibited to drive outward actin extension.
HostingRepository	PRIDE
AnnounceDate	2023-11-27
AnnouncementXML	Submission_2023-11-27_07:48:47.422.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	MARIETTE MATONDO
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-19 05:10:14	ID requested
⏵ 1	2023-11-27 07:48:47	announced

10.15252/EMBJ.2023113761;

submitter keyword: GFP-Trap,TNT

Michael Henderson
contact affiliation	Unite de Trafic membranaire et Pathogenese, Chiara Zurzolo Lab, Institut Pasteur
contact email	michael.henderson@pasteur.fr
lab head
MARIETTE MATONDO
contact affiliation	Pasteur
contact email	mariette.matondo@pasteur.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/11/PXD034649

PRIDE project URI

• PRIDE
  1. PXD034649
     1. Label: PRIDE project
     2. Name: Eps8, Arp2/3 and IRSp53 in TNT formation