PXD034638

PXD034638 is an original dataset announced via ProteomeXchange.

Title	Spermidine reduces neuroinflammation and soluble amyloid beta in an Alzheimer’s disease mouse model
Description	Deposition of amyloid beta (Aβ) and hyperphosphorylated tau along with glial cell-mediated neuroinflammation are prominent pathogenic hallmarks of Alzheimer’s disease (AD). In recent years, impairment of autophagy has been found to be another important feature contributing to AD progression. Therefore, the potential of the autophagy activator spermidine, a small body-endogenous polyamine often used as dietary supplement, was assessed on Aβ pathology and glial cell-mediated neuroinflammation. Oral treatment of the amyloid prone AD-like APPPS1 mice with spermidine reduced neurotoxic soluble Aβ and decreased AD-associated neuroinflammation. A subsequent proteome analysis of isolated microglia confirmed the anti-inflammatory and revealed cytoskeletal effects of spermidine in APPPS1 mice. Our data highlight that the autophagy activator spermidine holds the potential to enhance Aβ degradation and to counteract microglia-mediated neuroinflammation in AD pathology.
HostingRepository	PRIDE
AnnounceDate	2022-07-04
AnnouncementXML	Submission_2022-07-04_04:07:07.024.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Michael Mülleder
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-19 04:38:06	ID requested
⏵ 1	2022-07-04 04:07:07	announced

Freitag K, Sterczyk N, Wendlinger S, Obermayer B, Schulz J, Farztdinov V, M, ü, lleder M, Ralser M, Houtman J, Fleck L, Braeuning C, Sansevrino R, Hoffmann C, Milovanovic D, Sigrist SJ, Conrad T, Beule D, Heppner FL, Jendrach M, Spermidine reduces neuroinflammation and soluble amyloid beta in an Alzheimer's disease mouse model. J Neuroinflammation, 19(1):172(2022) [pubmed]

submitter keyword: Alzheimer’s disease, neuroinflammation, microglia, autophagy, spermidine, dietary supplement, liquid chromatography tandem mass spectrometry, phagocytosis

Frank Heppner
contact affiliation	Department of Neuropathology, Charité – Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, Berlin Institute of Health, Berlin, Germany
contact email	frank.heppner@charite.de
lab head
Michael Mülleder
contact affiliation	Core Facility -High-Throughput Mass Spectrometry, Charité -Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin and Berlin Institute of Health (BIH), Berlin, Germany
contact email	michael.muelleder@charite.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034638
     1. Label: PRIDE project
     2. Name: Spermidine reduces neuroinflammation and soluble amyloid beta in an Alzheimer’s disease mouse model