PXD034629

PXD034629 is an original dataset announced via ProteomeXchange.

Title	Mass spectrometry-driven discovery of neuropeptides mediating nictation behavior of nematodes
Description	Very little is known about the genetic modulation of nictation, a host-finding strategy used by infective juveniles of many pathogenic nematodes, as well as a phoretic strategy of Caenorhabditis elegans dauers. We here use a mass spectrometry-driven approach to find neuropeptides involved in the modulation of this behavior. We identified 126 neuropeptides in infective juveniles of the commercially relevant entomopathogenic nematode Steinernema carpocapsae, 75 of which have orthologs in the model organism C. elegans. To prioritize candidates for causality testing in light of the stage-restricted nictation behavior, we developed quantitative peptidomic assays and carried out targeted proteomic measurements that allows comparing neuropeptide levels at different C. elegans life stages. Our results show that virtually all 164 quantified neuropeptides are more abundant in dauers compared to L3 juveniles. Even so, not all are relevant to nictation, and we identify the neuropeptide genes flp-7 and flp-11 as novel regulators of nictation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:15.672.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034629
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sven Van Bael
SpeciesList	scientific name: Steinernema carpocapsae; NCBI TaxID: 34508;
ModificationList	amidated residue; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-17 08:07:18	ID requested
1	2022-12-08 04:27:24	announced
⏵ 2	2023-11-14 08:57:16	announced	2023-11-14: Updated project metadata.

10.6019/PXD034629;

submitter keyword: Nictation, Data-dependent acquisition, Steinernema Carpocapsae,Peptidomics, Parallel reaction monitoring, Caenorhabditis elegans

Liesbet Temmerman
contact affiliation	Laboratory of Molecular and Functional Neurobiology (Temmerman Lab), Department of Biology, KU Leuven, Belgium
contact email	Liesbet.Temmerman@kuleuven.be
lab head
Sven Van Bael
contact affiliation	KU Leuven
contact email	sven.vanbael@kuleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034629
     1. Label: PRIDE project
     2. Name: Mass spectrometry-driven discovery of neuropeptides mediating nictation behavior of nematodes