PXD034620 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients |
Description | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection not only exerts a deep impact over the respiratory system, but it also affects the cardiovascular system, causing vascular damage and thromboembolic events in critical patients. The endothelial dysfunction taking place in response to COVID-19 represents one of the first steps in this cascade of events that might leads to long-term sequelae over the vascular function. However, despite the enormous efforts in the last two years, the molecular mechanisms involved in such processes remains poorly understood. Using a label free-quantitative proteomics approach, we profiled the proteome of endothelial colony forming cells (ECFCs), after its incubation with the serum from individuals infected with COVID-19, whether asymptomatic or critical patients. Specifically, ECFCs were incubated ex-vivo with the serum of either SARS-CoV-2 negative donors (PCR-/IgG-), SARS-CoV-2 positive asymptomatic donors, at different infective stages: PCR+/ IgG- and PCR-/IgG+, or hospitalized critical COVID-19 patients, followed by mass spectrometry analysis. Remarkably, in response to all infected serums, the differentially expressed protein were found to be correlated with alterations in viral infection, RNA metabolism or autophagy, among others. Moreover, the serum of critical patients promoted the highest number of protein alterations, many of which associated with cardiovascular related pathologies. Finally, machine learning algorithms confirmed the discriminating potential of some proteins differentially expressed between conditions. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:56:21.159.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Santosh Bhosale |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-17 07:25:17 | ID requested | |
1 | 2023-03-20 02:52:31 | announced | |
2 | 2023-04-06 03:38:29 | announced | 2023-04-06: Updated project metadata. |
⏵ 3 | 2023-11-14 08:56:21 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
ProteomeXchange project tag: Sars-cov-2, Covid-19 |
submitter keyword: COVID-19, Viral infection, Mass spectrometry, RNA metabolism, ECFCs |
Contact List
Martin Røssel Larsen |
contact affiliation | Professor, Department of Biochemistry and Molecular Biology, University of Southern Denmark (SDU), Denmark |
contact email | mrl@bmb.sdu.dk |
lab head | |
Santosh Bhosale |
contact affiliation | Postdoc |
contact email | santoshb@bmb.sdu.dk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034620
- Label: PRIDE project
- Name: Cardiovascular-related proteomic changes in ECFCs exposed to the serum of COVID-19 patients