PXD034615
PXD034615 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | BRD4 regulates the osteogenic differentiation of MSCs |
| Description | MSCs were lysed in cell lysis buffer for Western blotting and IP (Beyotime, Cat. No. P0013). A Dynabeads™ protein G immunoprecipitation kit (Invitrogen, Cat. No. 10007D) was used for Co-IP according to the manufacturer’s instructions. Dynabeads were resuspended and placed on a magnet to remove the supernatant then incubated with Ab Binding & Washing Buffer containing an anti-BRD4 antibody for 10 min at room temperature with rotation. The supernatant was removed by placing tubes on a magnet, and the Dynabeads-antibody complex was washed with Ab Binding & Washing Buffer. MSC lysates were added to the Dynabeads-antibody mixture and incubated for 10 min at room temperature with rotation. The supernatant was removed, the Dynabeads-antibody complex mixture was washed with washing buffer, and the proteins were separated using a standard SDS–PAGE system. |
| HostingRepository | iProX |
| AnnounceDate | 2022-06-16 |
| AnnouncementXML | Submission_2023-06-11_20:13:23.320.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Wenhui Yu |
| SpeciesList | scientific name: Homo sapiens; NCBI TaxID: 9606; |
| ModificationList | No PTMs are included in the dataset |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-06-16 18:45:28 | ID requested | |
| 1 | 2023-01-03 01:46:59 | announced | |
| ⏵ 2 | 2023-06-11 20:13:24 | announced | 2023-06-12: Update publication information. |
Publication List
| Yu W, Xie Z, Li J, Lin J, Su Z, Che Y, Ye F, Zhang Z, Xu P, Zeng Y, Xu X, Li Z, Feng P, Mi R, Wu Y, Shen H, Super enhancers targeting ZBTB16 in osteogenesis protect against osteoporosis. Bone Res, 11(1):30(2023) [pubmed] |
Keyword List
| submitter keyword: MSCs |
Contact List
| Huiyong Shen | |
|---|---|
| contact affiliation | The Eighth Affiliated Hospital of Sun Yat-sen University |
| contact email | shenhuiy@mail.sysu.edu.cn |
| lab head | |
| Wenhui Yu | |
| contact affiliation | The Eighth Affiliated Hospital of Sun Yat-sen University |
| contact email | yuwh5@mail2.sysu.edu.cn |
| dataset submitter | |
Full Dataset Link List
| iProX dataset URI |
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