PXD034606

PXD034606 is an original dataset announced via ProteomeXchange.

Title	A peptide-centric local stability assay enables proteome-scale identification of the protein targets of diverse ligands
Description	We found that ligand-induced protein local stability shifts can be sensitively detected on a proteome-wide scale by a limited proteolysis strategy using a large amount of trypsin to generate and analyze peptides directly from native proteins. This enabled the development of the “peptide-centric local stability assay” (PELSA), a modification-free approach that achieves unprecedented sensitivity in proteome-wide target identification and binding-region determination. We demonstrate the versatility of PELSA by investigating the interactions across various biological contexts.
HostingRepository	PRIDE
AnnounceDate	2024-11-27
AnnouncementXML	Submission_2024-11-26_21:06:35.588.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	KEJIA LI
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-16 03:56:43	ID requested
⏵ 1	2024-11-26 21:06:36	announced

10.1038/S41592-024-02553-7;

submitter keyword: Human, DIA, Exploris 480, LC-MS/MS

Mingliang Ye
contact affiliation	CAS Key Lab of Separation Sciences for Analytical Chemistry, National Chromatographic R&A Center, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
contact email	mingliang@dicp.ac.cn
lab head
KEJIA LI
contact affiliation	Dalian Institute of Chemical Physics, Chinese Academy of Sciences
contact email	lkj2016@dicp.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/11/PXD034606

PRIDE project URI

• PRIDE
  1. PXD034606
     1. Label: PRIDE project
     2. Name: A peptide-centric local stability assay enables proteome-scale identification of the protein targets of diverse ligands