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PXD034597

PXD034597 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleXenografted Tumor decellularization provides cell-specific in vitro 3D environment
DescriptionIn vitro cell culture studies are common in cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Methods: Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with human triple negative breast cancer (TNBC)c ells and cell behavior was compared among them and with other control 2D and 3D cell culture methods. Results: A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TMBC xenograft models had different extracellular matrix (ECM) composition compared to the rest of xenograft tumors tested. The in vitro re-cellularization of decellularized ECM (dECM) yields tumor-type specific cell-behavior in TNBC context. Conclusions: Data indicate that dECM derived from xenograft tumors is a feasible substrate for re-seeding purposes, thereby promoting tumor-type specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models.
HostingRepositoryPRIDE
AnnounceDate2022-09-12
AnnouncementXMLSubmission_2022-09-12_02:35:31.401.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMikel Azkargorta
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentmaXis
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-16 03:38:30ID requested
12022-09-12 02:35:32announced
Publication List
Iazzolino G, Mendibil U, Arnaiz B, Ruiz-de-Angulo A, Azkargorta M, Uribe KB, Khatami N, Elortza F, Olalde B, Gomez-Vallejo V, Llop J, Abarrategi A, 3D environment. Front Oncol, 12():956940(2022) [pubmed]
Keyword List
submitter keyword: Tumor Xenograft
decellularization
in vitro 3D model
Breast cancer
Contact List
Felix Elortza
contact affiliationProteomics Platform CIC bioGUNE Bizkaia Tech. Park Build. 800 48160 Derio Spain
contact emailfelortza@cicbiogune.es
lab head
Mikel Azkargorta
contact affiliationProteomics Platform CIC bioGUNE
contact emailmazkargorta@cicbiogune.es
dataset submitter
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Dataset FTP location
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