PXD034597 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Xenografted Tumor decellularization provides cell-specific in vitro 3D environment |
Description | In vitro cell culture studies are common in cancer research field, and reliable biomimetic 3D models are needed to ensure physiological relevance. In this manuscript, we hypothesized that decellularized xenograft tumors can serve as an optimal 3D substrate to generate a top-down approach for in vitro tumor modeling. Methods: Multiple tumor cell lines were xenografted and the formed solid tumors were recovered for their decellularization by several techniques and further characterization by histology and proteomics techniques. Selected decellularized tumor xenograft samples were seeded with human triple negative breast cancer (TNBC)c ells and cell behavior was compared among them and with other control 2D and 3D cell culture methods. Results: A soft treatment using Freeze-EDTA-DNAse allows proper decellularization of xenografted tumor samples. Interestingly, proteomic data show that samples decellularized from TMBC xenograft models had different extracellular matrix (ECM) composition compared to the rest of xenograft tumors tested. The in vitro re-cellularization of decellularized ECM (dECM) yields tumor-type specific cell-behavior in TNBC context. Conclusions: Data indicate that dECM derived from xenograft tumors is a feasible substrate for re-seeding purposes, thereby promoting tumor-type specific cell behavior. These data serve as a proof-of-concept for further potential generation of patient-specific in vitro research models. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-12 |
AnnouncementXML | Submission_2022-09-12_02:35:31.401.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Mikel Azkargorta |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | maXis |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-16 03:38:30 | ID requested | |
⏵ 1 | 2022-09-12 02:35:32 | announced | |
Publication List
Iazzolino G, Mendibil U, Arnaiz B, Ruiz-de-Angulo A, Azkargorta M, Uribe KB, Khatami N, Elortza F, Olalde B, Gomez-Vallejo V, Llop J, Abarrategi A, 3D environment. Front Oncol, 12():956940(2022) [pubmed] |
Keyword List
submitter keyword: Tumor Xenograft |
decellularization |
in vitro 3D model |
Breast cancer |
Contact List
Felix Elortza |
contact affiliation | Proteomics Platform CIC bioGUNE Bizkaia Tech. Park Build. 800 48160 Derio Spain |
contact email | felortza@cicbiogune.es |
lab head | |
Mikel Azkargorta |
contact affiliation | Proteomics Platform CIC bioGUNE |
contact email | mazkargorta@cicbiogune.es |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034597
- Label: PRIDE project
- Name: Xenografted Tumor decellularization provides cell-specific in vitro 3D environment