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PXD034583

PXD034583 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleADR-directly-interfering amino acids based on competitive isoTOP-ABPP in normoxia
DescriptionHepatocellular carcinoma (HCC) is one of the most malignant tumors in the world with high morbidity and mortality rate. Many strategies have been tried to fight with HCC, yet effective treatment is still lacking. In this study, the in vitro and in vivo anti-HCC activity of andrographolide (ADR), an active ingredient of Andrographis Herba, has been demonstrated. We performed quantitative chemoproteomic profiling and identified heat shock protein HSP 90-alpha (HSP90AA1), an important molecular chaperone in endoplasmic reticulum protein processing, as the key target of ADR. The compound covalently bound to C597 and C598 therefore directly inhibited HSP90AA1 with isoform selectivity and reduced protein synthesis and triggered mitochondrial pathway-mediated apoptosis in HCC cancer cells collected in normoxia. Moreover, the inhibitory ability of ADR to HSP90AA1 was negatively correlated with the amount of this protein in cancer cells, and the mechanism was different from the existing inhibitors. Our study of ADR as a naturally derived and selective HSP90AA1 inhibitor provides new ideas for the treatment of HCC as well as other cancers.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:35:42.163.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterwei zhou
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdeaminated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-16 02:42:08ID requested
12023-03-10 19:25:33announced
22023-11-14 08:35:43announced2023-11-14: Updated project metadata.
Publication List
Mehta S, Carvalho VM, Rajczewski AT, Pible O, Gr, ΓΌ, ning BA, Johnson JE, Wagner R, Armengaud J, Griffin TJ, Jagtap PD, Catching the Wave: Detecting Strain-Specific SARS-CoV-2 Peptides in Clinical Samples Collected during Infection Waves from Diverse Geographical Locations. Viruses, 14(10):(2022) [pubmed]
Keyword List
submitter keyword: andrographolide
Contact List
Jianye Dai
contact affiliationSchool of Pharmacy,Lanzhou University
contact emaildaijy@lzu.edu.cn
lab head
wei zhou
contact affiliationLanzhou University
contact email1370882142@qq.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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