PXD034577

PXD034577 is an original dataset announced via ProteomeXchange.

Title	Characterization of the proteome of human cytochrome c oxidase assembly factors - Experiment D
Description	Mitochondrial cytochrome c oxidase (CcO) or respiratory chain complex IV is a heme aa3-copper oxygen reductase containing metal centers essential for holo-complex biogenesis and enzymatic function that are assembled by subunit-specific metallochaperones. The enzyme has two copper sites located in the catalytic core subunits. The COX1 subunit harbors the CuB site that tightly associates with heme a3 while the COX2 subunit contains the binuclear CuA site. Here, we report that in human cells the CcO copper chaperones form macromolecular assemblies and cooperate with several twin CX9C proteins to control heme a biosynthesis and coordinate copper transfer sequentially to the CuA and CuB sites. These data on CcO illustrate a mechanism that regulates the biogenesis of macromolecular enzymatic assemblies with several catalytic metal redox centers and prevents the accumulation of cytotoxic reactive assembly intermediates We characterized the proteome of COX11, COX19 and PET191 by AP + LC-MS-MS
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_23:46:30.553.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034577
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Antonio Barrientos
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-16 02:40:00	ID requested
⏵ 1	2022-08-11 23:46:31	announced

N, ý, vltov, á E, Dietz JV, Seravalli J, Khalimonchuk O, Barrientos A, Coordination of metal center biogenesis in human cytochrome c oxidase. Nat Commun, 13(1):3615(2022) [pubmed]

submitter keyword: mitochondria, cytochrome c oxidase, COX11, COX19, PET191

Antoni Barrientos
contact affiliation	University of Miami School of Medicine
contact email	abarrientos@med.miami.edu
lab head
Antonio Barrientos
contact affiliation	University of Miami School of Medicine
contact email	abarrientos@med.miami.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034577
     1. Label: PRIDE project
     2. Name: Characterization of the proteome of human cytochrome c oxidase assembly factors - Experiment D