PXD034564

PXD034564 is an original dataset announced via ProteomeXchange.

Title	Reduced polymerase pausing compensates for increased chromatin accessibility in the aging liver
Description	Regulation of gene expression is linked to the organization of the genome. With age, chromatin alterations occur on all levels of genome organization, accompanied by changes in the gene expression profile. However, little is known about the changes on the level of transcriptional regulation. Here, we used a multi-omics approach and integrated ATAC-, RNA- and NET-seq to identify age-related changes in the chromatin landscape of murine liver and to investigate how these are linked to transcriptional regulation. We provide the first systematic inventory of the connection between aging, chromatin accessibility and transcriptional regulation in a whole tissue. Aging in murine liver is characterized by an increase in chromatin accessibility at promoter regions, but not in an increase of transcriptional output. Instead, aging is accompanied by a decrease of promoter-proximal pausing of RNA polymerase II (Pol II). We propose that these changes in transcriptional regulation are due to a reduced stability of the pausing complex and may represent a mechanism to compensate for the age-related increase in chromatin accessibility in order to prevent aberrant transcription.
HostingRepository	PRIDE
AnnounceDate	2022-09-15
AnnouncementXML	Submission_2022-09-15_03:15:38.494.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ilian Atanassov
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-16 02:23:58	ID requested
⏵ 1	2022-09-15 03:15:40	announced

Bozukova M, Nikopoulou C, Kleinenkuhnen N, Grbavac D, Goetsch K, Tessarz P, Aging is associated with increased chromatin accessibility and reduced polymerase pausing in liver. Mol Syst Biol, 18(9):e11002(2022) [pubmed]

submitter keyword: polymerase, chromating, aging, liver, TMT

Peter Tessarz
contact affiliation	Max Planck Institute for Biology of Ageing, Joseph-Stelzmann-Str. 9b, 50931 Cologne, Germany CECAD Research Center, Joseph-Stelzmann-Str. 26, 50931 Cologne, Germany
contact email	ptessarz@age.mpg.de
lab head
Ilian Atanassov
contact affiliation	Max Planck Institute for Biology of Aging
contact email	Ilian.Atanassov@age.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD034564

PRIDE project URI

• PRIDE
  1. PXD034564
     1. Label: PRIDE project
     2. Name: Reduced polymerase pausing compensates for increased chromatin accessibility in the aging liver