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PXD034531

PXD034531 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAlveolar epithelial cells are competent producers of interstitial extracellular matrix with disease relevant plasticity in a human in vitro 3D model
DescriptionThe structure of alveoli is critical for proper lung function, the extracellular matrix (ECM) that forms these delicate structures need to be maintained throughout life, when this fails, patients suffer from conditions such as emphysema or lung fibrosis. The alveolar walls are lined by alveolar epithelial cells (AEC), and we herein set out to examine their potential to contribute to ECM remodeling in a human three-dimensional in vitro model based on human lung ECM. Cryopreserved type 2 AEC (AEC2) isolated from healthy lungs and lungs of patients with chronic obstructive pulmonary disease (COPD) were cultured in decellularized human lung slices over a period of 13 days. AEC2 from healthy lungs were treated with transforming growth factor ß1 (TGF-ß1) to evaluate the plasticity of their ECM production. Evaluation of phenotypic markers and expression of matrisome genes and proteins were performed by RNA-sequencing, mass spectrometry and immunohistochemistry. AEC2 in our model displayed an AEC marker profile similar to freshly isolated AEC2. COPD-derived AEC2 retained expression of known disease markers such as HLA-A throughout the culture period. AEC2 matrisome expression was found not to be limited to basement membrane components but included a complex set of structural proteins found in interstitial ECM. With TGF-ß1 stimuli, AEC2 showed a change in ECM production resembling what have previously been documented in mesenchymal cells, without loss of AEC marker expression. A previously unexplored potential of AEC to directly contribute to ECM turnover is revealed, motivating a re-evaluation of the role of AEC2 in pathological lung remodeling.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_05:51:24.836.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD034531
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterOskar Rosmark
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmethionine oxidation with neutral loss of 64 Da; 4-hydroxy-L-proline; acetylated residue; deamidated L-asparagine; iodoacetamide derivatized residue
InstrumentQ Exactive HF-X
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-15 03:40:01ID requested
12023-06-29 08:19:13announced
22023-11-14 09:01:43announced2023-11-14: Updated project metadata.
32024-10-22 05:51:25announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41598-023-35011-z;
Rosmark O, Kadefors M, Dellgren G, Karlsson C, Ericsson A, Lindstedt S, Malmstr, ö, m J, Hallgren O, Larsson-Callerfelt AK, Westergren-Thorsson G, Alveolar epithelial cells are competent producers of interstitial extracellular matrix with disease relevant plasticity in a human in vitro 3D model. Sci Rep, 13(1):8801(2023) [pubmed]
10.6019/PXD034531;
Keyword List
submitter keyword: Human, ECM, IPF, COPD, Lung, Remodeling, Alveolar epithelium
Contact List
Gunilla Westergren-Thorsson
contact affiliationLung Biology, Department of Experimental Medical Science, Lund University, Lund, Sweden.
contact emailgunilla.westergren-thorsson@med.lu.se
lab head
Oskar Rosmark
contact affiliationLung Biology, Department Experimental Medical Science, Lund University, Lund, Sweden.
contact emailoskar.rosmark@med.lu.se
dataset submitter
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Dataset FTP location
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