PXD034531

PXD034531 is an original dataset announced via ProteomeXchange.

Title	Alveolar epithelial cells are competent producers of interstitial extracellular matrix with disease relevant plasticity in a human in vitro 3D model
Description	The structure of alveoli is critical for proper lung function, the extracellular matrix (ECM) that forms these delicate structures need to be maintained throughout life, when this fails, patients suffer from conditions such as emphysema or lung fibrosis. The alveolar walls are lined by alveolar epithelial cells (AEC), and we herein set out to examine their potential to contribute to ECM remodeling in a human three-dimensional in vitro model based on human lung ECM. Cryopreserved type 2 AEC (AEC2) isolated from healthy lungs and lungs of patients with chronic obstructive pulmonary disease (COPD) were cultured in decellularized human lung slices over a period of 13 days. AEC2 from healthy lungs were treated with transforming growth factor ß1 (TGF-ß1) to evaluate the plasticity of their ECM production. Evaluation of phenotypic markers and expression of matrisome genes and proteins were performed by RNA-sequencing, mass spectrometry and immunohistochemistry. AEC2 in our model displayed an AEC marker profile similar to freshly isolated AEC2. COPD-derived AEC2 retained expression of known disease markers such as HLA-A throughout the culture period. AEC2 matrisome expression was found not to be limited to basement membrane components but included a complex set of structural proteins found in interstitial ECM. With TGF-ß1 stimuli, AEC2 showed a change in ECM production resembling what have previously been documented in mesenchymal cells, without loss of AEC marker expression. A previously unexplored potential of AEC to directly contribute to ECM turnover is revealed, motivating a re-evaluation of the role of AEC2 in pathological lung remodeling.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:51:24.836.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034531
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Oskar Rosmark
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	methionine oxidation with neutral loss of 64 Da; 4-hydroxy-L-proline; acetylated residue; deamidated L-asparagine; iodoacetamide derivatized residue
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-15 03:40:01	ID requested
1	2023-06-29 08:19:13	announced
2	2023-11-14 09:01:43	announced	2023-11-14: Updated project metadata.
⏵ 3	2024-10-22 05:51:25	announced	2024-10-22: Updated project metadata.

10.1038/s41598-023-35011-z;

Rosmark O, Kadefors M, Dellgren G, Karlsson C, Ericsson A, Lindstedt S, Malmstr, ö, m J, Hallgren O, Larsson-Callerfelt AK, Westergren-Thorsson G, Alveolar epithelial cells are competent producers of interstitial extracellular matrix with disease relevant plasticity in a human in vitro 3D model. Sci Rep, 13(1):8801(2023) [pubmed]

10.6019/PXD034531;

submitter keyword: Human, ECM, IPF, COPD, Lung, Remodeling, Alveolar epithelium

Gunilla Westergren-Thorsson
contact affiliation	Lung Biology, Department of Experimental Medical Science, Lund University, Lund, Sweden.
contact email	gunilla.westergren-thorsson@med.lu.se
lab head
Oskar Rosmark
contact affiliation	Lung Biology, Department Experimental Medical Science, Lund University, Lund, Sweden.
contact email	oskar.rosmark@med.lu.se
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD034531

PRIDE project URI

• PRIDE
  1. PXD034531
     1. Label: PRIDE project
     2. Name: Alveolar epithelial cells are competent producers of interstitial extracellular matrix with disease relevant plasticity in a human in vitro 3D model