PXD034530

PXD034530 is an original dataset announced via ProteomeXchange.

Title	Chlorhexidine promotes psl in Pseudomonas aeruginosa that enhances cell aggregation with preserved pathogenicity demonstrates an adaptation against antiseptic
Description	Because Pseudomonas aeruginosa is a common pathogen that frequently contacts with Chlorhexidine digluconate (a regular antiseptic), then adaptations against Chlorhexidine were tested. In com-parison with the parent strain, the Chlorhexidine-adapted strain formed smaller colonies with the downregulation of several metabolic pathways (proteomic analysis) and increased resistance against colistin (an antibiotic for the current antibiotic-resistant bacteria), partly through the modification of L-Ara4N in the lipopolysaccharide at the outer membrane (proteomic analysis). Chlorhexidine-adapted strain formed dense liquid-solid interface biofilm with cell aggregation partly due to the Chlorhexidine-induced overexpression of psl (exopolysaccharide-encoded gene) through LadS/GacSA pathway (c-di-GMP-independence) in 12 h biofilms and maintained the ag-gregation with SiaD-mediated c-di-GMP dependence in 24 h biofilms as evaluated by polymerase chain reaction (PCR). The addition of Ca2+ in the Chlorhexidine-adapted strain facilitated several Psl-associated genes, indicating an impact of Ca2+ in Psl production. The sessile Chlorhexi-dine-treated bacteria demonstrated a lower expression of IL-6 and IL-8 on fibroblasts and mac-rophages than the parent strain, indicating less inflammatory reactions from the Chlorhexidine use. However, the Chlorhexidine-treated bacteria induces similar severity in 14 days of mouse wounds as indicated by wound score and bacterial burdens. In conclusion, Chlorhexidine induced psl over-expression and colistin cross-resistance that might be clinically important.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:39:37.827.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiradej Makjaroen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-15 03:39:39	ID requested
1	2022-10-13 16:55:11	announced
⏵ 2	2023-11-14 07:39:40	announced	2023-11-14: Updated project metadata.

Singkham-In U, Phuengmaung P, Makjaroen J, Saisorn W, Bhunyakarnjanarat T, Chatsuwan T, Chirathaworn C, Chancharoenthana W, Leelahavanichkul A, That Enhances Cell Aggregation with Preserved Pathogenicity Demonstrates an Adaptation against Antiseptic. Int J Mol Sci, 23(15):(2022) [pubmed]

submitter keyword: Pseudomonas aeruginosa

Chlorhexidine digluconate

Chlorhexidine

Cross-resistance

Biofilm

Psl

Cell aggregate

Persistence

wound infection

Asada Leelahavanichkul
contact affiliation	Translational Research in Inflammation and Immunology Research Unit (TRIRU), Department of Mi-crobiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok 10330, Thailand
contact email	aleelahavanit@gmail.com
lab head
Jiradej Makjaroen
contact affiliation	Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330
contact email	jiradejmak@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034530

PRIDE project URI

• PRIDE
  1. PXD034530
     1. Label: PRIDE project
     2. Name: Chlorhexidine promotes psl in Pseudomonas aeruginosa that enhances cell aggregation with preserved pathogenicity demonstrates an adaptation against antiseptic