PXD034528

PXD034528 is an original dataset announced via ProteomeXchange.

Title	The architecture and pathogenesis of Pseudomonas-Candida biofilms in infections and their influence on the microenvironment
Description	Interkingdom Pseudomonas-Candida biofilms is a human pathogen associated with acute and chronic infection. This study examine hoe their structural organization influences their in vivo microenvironment, which in turns affects the interaction of Pseudomonas-Candida biofilms with host immune responses. NCI-H292 grown P. aeruginosa biofilms with and without C. albicans biofilms were used for proteomic analysis. Biofilms were demonstrated in all groups which have 357 proteins from proteomic analysis including i) proteins in PA+CA were higher than PA (76 proteins, such as sigma factor AlgU negative regulatory protein; MucA) and ii) proteins in PA+CA were lower than PA (281 proteins, including alginate genes such as Alginate biosynthesis transcriptional regulatory protein; AlgB). Although most of Pseudomonas proteins in PA+CA were lower than PA due to the lower initial bacteria in preparation of mixed-organism biofilms, mucA overexpressed in PA+CA indicating that high alginate production that transcriptionally controlled by algU-mucABCD genes. These results demonstrated that Pseudomonas-Candida biofilms produced alginate more compared to Pseudomonas biofilms on lungs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_07:38:35.586.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiradej Makjaroen
SpeciesList	scientific name: Candida albicans (Yeast); NCBI TaxID: 5476;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-15 03:21:02	ID requested
1	2022-10-13 16:48:48	announced
⏵ 2	2023-11-14 07:38:39	announced	2023-11-14: Updated project metadata.

Phuengmaung P, Mekjaroen J, Saisorn W, Chatsuwan T, Somparn P, Leelahavanichkul A, on the Pulmonary Cell Surface and in Mice, a Possible Cause of Chronic Mixed Organismal Lung Lesions. Int J Mol Sci, 23(16):(2022) [pubmed]

submitter keyword: Pseudompnas aeruginosa, Interkingdom biofilms, Pneumonia, Candida albicans

Asada Leelahavanichkul
contact affiliation	Translational Research in Inflammation and Immunology Research Unit (TRIRU), Department of Mi-crobiology, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Road, Pathumwan, Bangkok 10330, Thailand
contact email	aleelahavanit@gmail.com
lab head
Jiradej Makjaroen
contact affiliation	Center of Excellence in Systems Biology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330
contact email	jiradejmak@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034528

PRIDE project URI

• PRIDE
  1. PXD034528
     1. Label: PRIDE project
     2. Name: The architecture and pathogenesis of Pseudomonas-Candida biofilms in infections and their influence on the microenvironment