PXD034516

PXD034516 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	SILAC Phospho-proteomics: Trypanosoma brucei
Description	Pseudokinases can modulate activity of protein kinases, among other modes of influencing biological pathways. We tested a hypothesis that knockdown of Tb427tmp.160.4770 (a Pseudokinase) perturbs the Phospho-proteome of T. brucei. To discover changes in the trypanosome phospho-proteome, stable isotope labelling of amino acids in cell culture (SILAC) was performed, followed by enrichment of phosphopeptides with immobilised metal affinity chromatography (IMAC), and phospho-peptide detection/quantitation using mass spectroscopy. Trypanosomes (p2T7-Tb4770) were cultured in heavy (13C6-L-Arginine, 2H4-L-Lysine) or light isotopes (L-Arginine, L-Lysine) for SILAC. Trypanosomes grown in heavy medium were induced with tetracycline for knockdown of Tb427tmp.160.4770. Induced (Tet+, H) and uninduced (Tet-, L) samples were combined and processed together for IMAC and mass spectroscopy
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:27:52.812.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gaurav Kumar
SpeciesList	scientific name: Trypanosoma brucei; NCBI TaxID: 5691;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-14 09:40:16	ID requested
1	2023-03-10 19:17:40	announced
⏵ 2	2023-11-14 08:27:56	announced	2023-11-14: Updated project metadata.

Publication List

Kumar G, Thomas B, Mensa-Wilmot K, Pseudokinase NRP1 facilitates endocytosis of transferrin in the African trypanosome. Sci Rep, 12(1):18572(2022) [pubmed]

Kumar G, Thomas B, Mensa-Wilmot K, Pseudokinase NRP1 facilitates endocytosis of transferrin in the African trypanosome. Sci Rep, 12(1):18572(2022) [pubmed]

Keyword List

submitter keyword: LC-MSMS, SILAC,Trypanosoma brucei

submitter keyword: LC-MSMS, SILAC,Trypanosoma brucei

Contact List

Kojo-Mensa Wilmot
contact affiliation	College of Science and Mathematics, Kennesaw State University, USA
contact email	kmensawi@kennesaw.edu
lab head
Gaurav Kumar
contact affiliation	Kennesaw State University
contact email	gkumar2@kennesaw.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034516
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034516

PRIDE project URI

Repository Record List

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