PXD034510

PXD034510 is an original dataset announced via ProteomeXchange.

Title	SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner
Description	Visceral adiposity is a risk factor for severe COVID-19, and a link between adipose tissue infection and disease progression has been proposed. Here we demonstrate that SARS-CoV-2 infects human adipose tissue and undergoes productive infection in human primary adipose-derived stromal-vascular cells differentiated into adipocytes. However, the permissiveness to infection and the cellular response depends on the anatomical origin of the cells and the viral lineage. Cells of visceral adipose tissue origin express more ACE2 and are more permissive to SARS-CoV-2 infection than their subcutaneous counterpart. SARS-CoV-2 infection leads to inhibition of lipolysis in cells of subcutaneous origin, while in visceral fat cells, it results in higher expression of pro-inflammatory cytokines. Viral load and cellular response are attenuated when visceral adipose tissue cells are infected with the SARS-CoV-2 gamma variant. A similar degree of cell death occurs 4-days after SARS-CoV-2 infection, regardless of the cell origin or viral lineage. Hence, SARS-CoV-2 infects human adipose tissue cells, replicating and altering cell function and viability in a fat depot- and viral lineage-dependent fashion.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:06:34.833.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034510
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Bradley Smith
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-14 09:24:48	ID requested
1	2022-10-14 06:28:24	announced
⏵ 2	2023-11-14 08:06:35	announced	2023-11-14: Updated project metadata.

Saccon TD, Mousovich-Neto F, Ludwig RG, Carregari VC, Dos Anjos Souza AB, Dos Passos ASC, Martini MC, Barbosa PP, de Souza GF, Muraro SP, Forato J, Amorim MR, Marques RE, Veras FP, Barreto E, Gon, ç, alves TT, Paiva IM, Fazolini NPB, Onodera CMK, Martins Junior RB, de Ara, ú, jo PHC, Batah SS, Viana RMM, de Melo DM, Fabro AT, Arruda E, Queiroz Cunha F, Cunha TM, Pretti MAM, Smith BJ, Marques-Souza H, Knittel TL, Ruiz GP, Profeta GS, Fontes-Cal TCM, Boroni M, Vinolo MAR, Farias AS, Moraes-Vieira PMM, Bizzacchi JMA, Teesalu T, Chaim FDM, Cazzo E, Chaim EA, Proen, ç, a-M, ó, dena JL, Martins-de-Souza D, Osako MK, Leiria LO, Mori MA, SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner. Nat Commun, 13(1):5722(2022) [pubmed]

10.6019/PXD034510;

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: lipolysis, inflammation, SARS-CoV-2, fat depots, adipose tissue, COVID-19,obesity, visceral fat, virus attenuation, variants of concern

Marcelo A. Mori
contact affiliation	Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil
contact email	morima@unicamp.br
lab head
Bradley Smith
contact affiliation	UNICAMP
contact email	a22brad22@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034510

PRIDE project URI

• PRIDE
  1. PXD034510
     1. Label: PRIDE project
     2. Name: SARS-CoV-2 infects adipose tissue in a fat depot- and viral lineage-dependent manner