PXD034483
PXD034483 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Proteins co-localized with RNA Pol2 in ALS patient-derived neural precursors |
| Description | Deficiency of proteostasis and RNA metabolism are increasingly recognized as critical drivers of neurodegenerative diseases. In this study, we focused on RNA polymerase II (Pol2) as the converging point of these two elements to understand how transcriptional machinery is affected by ALS-associated VCP mutations. Pol2 highly depends on its accessory factors to initiate, elongate, and terminate the transcription properly with accurate RNA splicing and processing. We, therefore, studied proteins co-localised with Pol2 in neural precursors obtained from ALS-patient iPS cells. To do that, we used the SPACE method as a highly sensitive proteomic tool to eliminate the artificial interactions during purification. |
| HostingRepository | jPOST |
| AnnounceDate | 2023-03-02 |
| AnnouncementXML | Submission_2023-03-02_09:00:44.790.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Mahmoudreza Rafiee |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; alpha-amino acetylated residue |
| Instrument | LTQ Orbitrap |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2022-06-11 15:43:29 | ID requested | |
| ⏵ 1 | 2023-03-02 09:00:45 | announced |
Publication List
| Rafiee MR, Rohban S, Davey K, Ule J, Luscombe NM, RNA polymerase II-associated proteins reveal pathways affected in VCP-related amyotrophic lateral sclerosis. Brain, 146(6):2547-2556(2023) [pubmed] |
Keyword List
| submitter keyword: RNA polymerase II, ALS, Chromatin, Proteostasis |
Contact List
| Nicholas Luscombe | |
|---|---|
| lab head | |
| Mahmoudreza Rafiee | |
| contact affiliation | The Crick |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST001663/ |
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