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PXD034476

PXD034476 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDeep Proteome profiling reveals signatures of age and sex differences in paw skin and sciatic nerve of naïve mice
DescriptionThe age and sex of studied animals profoundly impacts experimental outcomes in animal-based biomedical research. However, most preclinical studies in mice use a wide-spanning age range from 4 to 14 weeks and do not assess study parameters in male and female mice in parallel. This raises concerns regarding reproducibility and neglect of potentially relevant age and sex differences. Furthermore, the molecular setup of tissues in dependence of age and sex is unknown in naïve mice precluding efficient translational research. Here, we first compared two different mass spectrometric acquisition methods – DDA- and DIA-PASEF – in order to maximize the depth of proteome quantitation. We then employed an optimized workflow of quantitative proteomics based on DIA-PASEF followed by DIA-NN data analysis, and revealed significant differences in mouse paw skin and sciatic nerve (SCN) when comparing (i) male and female mice, and, in parallel, (ii) adolescent mice (4 weeks) with adult mice (14 weeks).
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:57:07.535.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFeng Xian
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListacetylated residue; monohydroxylated residue; deaminated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-10 08:24:16ID requested
12022-11-29 03:49:59announced
22023-11-14 08:57:08announced2023-11-14: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: mouse, dia-pasef, skin, sex, sciatic nerve, proteome, age
Contact List
Manuela Schmidt
contact affiliationSystems Biology of Pain,Division of Pharmacology & Toxicology, Department of Pharmaceutical Sciences, University of Vienna
contact emailmanuela_schmidt@univie.ac.at
lab head
Feng Xian
contact affiliationDivision of Pharmacology and Toxicology, Department of Pharmaceutical Sciences, University of Vienna
contact emailfeng.xian@univie.ac.at
dataset submitter
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Dataset FTP location
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PRIDE project URI
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