PXD034474

PXD034474 is an original dataset announced via ProteomeXchange.

Title	Characterization and composition of membrane vesicles and secreted proteins in Apilactobacillus kunkeei
Description	Secreted particles, including membrane vesicles, have increasingly been recognized as important for bacterial community functions and host-interaction processes, but their specific compositions and functional roles are debated. In this study, we have characterized the secreted particles of Apilactobacillus kunkeei, a defensive symbiont of honeybees. We cultivated A. kunkeei strains A1401 and A0901 and separated the secreted protein particles from the extracellular membrane vesicles using density gradient ultracentrifugation. A proteomics analyses identified more than 500 proteins in each strain, of which 27 to 45 proteins were relatively more abundant in the cell-free supernatant, including glycoside hydrolases and peptidases. The extracellular transcriptome associated with the membrane vesicles contained a relatively higher fraction of mRNAs derived from highly transcribed operons such as those for ribosomal proteins and ATP synthase subunits, whereas highly expressed tRNAs were relatively more abundant in the cellular fraction. Based on these results, we propose that mRNAs for highly expressed proteins are overproduced and that superfluous mRNAs are fragmented, packaged into membrane vesicles and secreted. The results have implications for the utilization of membrane vesicles in A. kunkeei as a delivery tool for small RNA molecules, while also providing more general insights into the role of membrane vesicles in bacteria.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:24:01.446.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Seeger
SpeciesList	scientific name: Apilactobacillus kunkeei; NCBI TaxID: 148814;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-10 08:21:36	ID requested
1	2023-09-07 11:54:26	announced
⏵ 2	2023-11-14 08:24:01	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: membrane vesicles,Apilactobacillus kunkeei, secreted proteins, lactic acid bacteria

Siv Andersson
contact affiliation	ICM, Molecular Evolution, Uppsala University
contact email	siv.andersson@icm.uu.se
lab head
Christian Seeger
contact affiliation	Molecular Evolution, Department of Cell and Molecular Biology, Science for Life Laboratory, Biomedical Centre, Uppsala University, SE-751 24 Uppsala, Sweden
contact email	christian.seeger@icm.uu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034474
     1. Label: PRIDE project
     2. Name: Characterization and composition of membrane vesicles and secreted proteins in Apilactobacillus kunkeei