PXD034473 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization and composition of membrane vesicles and secreted proteins in Apilactobacillus kunkeei |
Description | Secreted particles, including membrane vesicles, have increasingly been recognized as important for bacterial community functions and host-interaction processes, but their specific compositions and functional roles are debated. In this study, we have characterized the secreted particles of Apilactobacillus kunkeei, a defensive symbiont of honeybees. We cultivated A. kunkeei strains A1401 and A0901 and separated the secreted protein particles from the extracellular membrane vesicles using density gradient ultracentrifugation. A proteomics analyses identified more than 500 proteins in each strain, of which 27 to 45 proteins were relatively more abundant in the cell-free supernatant, including glycoside hydrolases and peptidases. The extracellular transcriptome associated with the membrane vesicles contained a relatively higher fraction of mRNAs derived from highly transcribed operons such as those for ribosomal proteins and ATP synthase subunits, whereas highly expressed tRNAs were relatively more abundant in the cellular fraction. Based on these results, we propose that mRNAs for highly expressed proteins are overproduced and that superfluous mRNAs are fragmented, packaged into membrane vesicles and secreted. The results have implications for the utilization of membrane vesicles in A. kunkeei as a delivery tool for small RNA molecules, while also providing more general insights into the role of membrane vesicles in bacteria. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:18:33.239.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christian Seeger |
SpeciesList | scientific name: Apilactobacillus kunkeei; NCBI TaxID: 148814; |
ModificationList | iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-10 08:18:46 | ID requested | |
1 | 2023-09-07 11:54:01 | announced | |
⏵ 2 | 2023-11-14 08:18:33 | announced | 2023-11-14: Updated project metadata. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: extracellular proteins, membrane vesicles,lactic acid bacteria, Apilactobacillus kunkeei |
Contact List
Siv Andersson |
contact affiliation | ICM, Molecular Evolution, Uppsala University |
contact email | siv.andersson@icm.uu.se |
lab head | |
Christian Seeger |
contact affiliation | Molecular Evolution, Department of Cell and Molecular Biology, Science for Life Laboratory, Biomedical Centre, Uppsala University, SE-751 24 Uppsala, Sweden |
contact email | christian.seeger@icm.uu.se |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034473
- Label: PRIDE project
- Name: Characterization and composition of membrane vesicles and secreted proteins in Apilactobacillus kunkeei