PXD034470

PXD034470 is an original dataset announced via ProteomeXchange.

Title	lncRNA HOTAIRM1 Coordinates with RNA Processing Factors in DNA Damage Repair
Description	The eukaryotic RNA processing factor Y14 participates in double-strand break (DSB) repair via its RNA-dependent interaction with the non-homologous end-joining (NHEJ) complex. We identified the long non-coding RNA HOTAIRM1 as a candidate that mediates this interaction. HOTAIRM1 localized to DNA damage sites induced by ionizing radiation. Depletion of HOTAIRM1 delayed the recruitment of DNA damage response and repair factors to DNA lesions and reduced DNA repair efficiency. Identification of the HOTAIRM1 interactome revealed a large set of RNA processing factors including mRNA surveillance factors. The surveillance factors Upf1 and SMG6 localized to DNA damage sites in a HOTAIRM1-dependent manner. Depletion of Upf1 or SMG6 increased the level of DSB-induced non-coding transcripts at damaged sites, indicating a pivotal role for Upf1/SMG6-mediated RNA degradation in DNA repair. We conclude that HOTAIRM1 serves as an assembly scaffold for both DNA repair and RNA processing factors that act in concert to repair DSBs.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:15.318.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Fu-An Li
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-10 03:31:54	ID requested
1	2023-05-10 13:16:02	announced
⏵ 2	2023-11-14 08:46:16	announced	2023-11-14: Updated project metadata.

Chuang TW, Su CH, Wu PY, Chang YM, Tarn WY, LncRNA HOTAIRM1 functions in DNA double-strand break repair via its association with DNA repair and mRNA surveillance factors. Nucleic Acids Res, 51(7):3166-3184(2023) [pubmed]

submitter keyword: LC/MS/MS based proteomics

Woan-Yuh Tarn
contact affiliation	Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan
contact email	wtarn@ibms.sinica.edu.tw
lab head
Fu-An Li
contact affiliation	Institute of Biomedical Sciences, Academia Sinica
contact email	falee@ibms.sinica.edu.tw
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD034470

PRIDE project URI

• PRIDE
  1. PXD034470
     1. Label: PRIDE project
     2. Name: lncRNA HOTAIRM1 Coordinates with RNA Processing Factors in DNA Damage Repair