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PXD034458

PXD034458 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleQuantitation of ATIs in Wheat by LC-MS/MS – LFQ Orbitrap
DescriptionA selection of ATIs was silenced in the common wheat cultivar Bobwhite and durum wheat cultivar Svevo by RNAi and gene editing, respectively, in order to reduce the amounts of ATIs. The controls and silenced lines were analyzed after digestion to peptides by LC-MS/MS with different approaches to evaluate changes in composition of ATIs. First, a targeted method with stable isotope dilution assay (SIDA) using labeled peptides as internal standards was applied. Additionally, four different approaches for relative quantitation were conducted, in detail, iTRAQ labeled and label free quantitation (LFQ) combined with data dependent acquisition (DDA) and data independent acquisition (DIA). Quantitation was performed manually (Skyline and MASCOT) and with different proteomics software tools (PLGS, MaxQuant, and PEAKS X Pro).
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_07:27:34.668.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSabrina Geisslitz
SpeciesList scientific name: Triticum durum; NCBI TaxID: NCBITaxon:4567; scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationListiodoacetamide derivatized residue
InstrumentExactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-10 02:58:01ID requested
12022-10-13 15:01:09announced
22023-11-14 07:27:35announced2023-11-14: Updated project metadata.
Publication List
Geisslitz S, Islam S, Buck L, Grunwald-Gruber C, Sestili F, Camerlengo F, Masci S, D'Amico S, Absolute and relative quantitation of amylase/trypsin-inhibitors by LC-MS/MS from wheat lines obtained by CRISPR-Cas9 and RNAi. Front Plant Sci, 13():974881(2022) [pubmed]
Keyword List
submitter keyword: Amylase/Trypsin Inhibitor
Wheat
CRSIPR-Cas9
RNAi;
Contact List
Katharina Scherf
contact affiliationDepartment of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany
contact emailkatharina.scherf@kit.edu
lab head
Sabrina Geisslitz
contact affiliationDepartment of Bioactive and Functional Food Chemistry, Institut of Applied Biosciences, Karlsruhe Institut of Technology
contact emailsabrina.geisslitz@kit.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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