PXD034457 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic Analysis of RAP (RNA Antisense Purification) isolated RNPs (RiboNucleotide Particles) harboring the novel lncRNA mimi |
Description | In neurons, mRNAs and associated RNA-binding proteins assemble into ribonucleoprotein (RNP) granules essential to regulate mRNA trafficking, local translation, and turnover. Dysregulation of RNA-protein condensation can disturb synaptic plasticity. We report that the novel lncRNA mimi is a constitutive and essential component of large cytoplasmic condensates (RNP granules) in fly neurons. In order to identify direct mimi RNA binders we employ RAP assisted purification of mimi RNPs subsequent to UV-crosslinking of adult fly brain tissue (non UV irradiated samples serve as control). Applying relative Max Quant LFQ quantification (Max LFQ) we carry out a differential proteomic analysis of mimi RNP complexes in UV-irradiated versus non irradiated fly brains. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:30:14.041.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Gerhard Mittler |
SpeciesList | scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227; |
ModificationList | monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive Plus |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-10 02:33:54 | ID requested | |
1 | 2022-10-12 06:51:34 | announced | |
⏵ 2 | 2023-11-14 08:30:15 | announced | 2023-11-14: Updated project metadata. |
Publication List
Grzejda D, Mach J, Schweizer JA, Hummel B, Rezansoff AM, Eggenhofer F, Panhale A, Lalioti ME, Cabezas Wallscheid N, Backofen R, Felsenberg J, Hilgers V, scaffolds neuronal granules to maintain nervous system maturity. Sci Adv, 8(39):eabo5578(2022) [pubmed] |
Keyword List
submitter keyword: ELAV |
Drosophila |
relative LFQ (label free quantification) quantitative proteomics;,RAP RNA protein complex isolation |
UV crosslinking |
fly brain |
neurons |
lncRNA mimi |
FNE |
RBP9 |
Contact List
Gerhard Mittler |
contact affiliation | Max Planck Institute of Immunobiology and Epigenetics Proteomics Unit Stuebeweg 51 79108 Freiburg, Germany |
contact email | mittler@ie-freiburg.mpg.de |
lab head | |
Gerhard Mittler |
contact affiliation | Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics |
contact email | mittler@ie-freiburg.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034457
- Label: PRIDE project
- Name: Proteomic Analysis of RAP (RNA Antisense Purification) isolated RNPs (RiboNucleotide Particles) harboring the novel lncRNA mimi