<<< Full experiment listing

PXD034457

PXD034457 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomic Analysis of RAP (RNA Antisense Purification) isolated RNPs (RiboNucleotide Particles) harboring the novel lncRNA mimi
DescriptionIn neurons, mRNAs and associated RNA-binding proteins assemble into ribonucleoprotein (RNP) granules essential to regulate mRNA trafficking, local translation, and turnover. Dysregulation of RNA-protein condensation can disturb synaptic plasticity. We report that the novel lncRNA mimi is a constitutive and essential component of large cytoplasmic condensates (RNP granules) in fly neurons. In order to identify direct mimi RNA binders we employ RAP assisted purification of mimi RNPs subsequent to UV-crosslinking of adult fly brain tissue (non UV irradiated samples serve as control). Applying relative Max Quant LFQ quantification (Max LFQ) we carry out a differential proteomic analysis of mimi RNP complexes in UV-irradiated versus non irradiated fly brains.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:30:14.041.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGerhard Mittler
SpeciesList scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationListmonohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-10 02:33:54ID requested
12022-10-12 06:51:34announced
22023-11-14 08:30:15announced2023-11-14: Updated project metadata.
Publication List
Grzejda D, Mach J, Schweizer JA, Hummel B, Rezansoff AM, Eggenhofer F, Panhale A, Lalioti ME, Cabezas Wallscheid N, Backofen R, Felsenberg J, Hilgers V, scaffolds neuronal granules to maintain nervous system maturity. Sci Adv, 8(39):eabo5578(2022) [pubmed]
Keyword List
submitter keyword: ELAV
Drosophila
relative LFQ (label free quantification) quantitative proteomics;,RAP RNA protein complex isolation
UV crosslinking
fly brain
neurons
lncRNA mimi
FNE
RBP9
Contact List
Gerhard Mittler
contact affiliationMax Planck Institute of Immunobiology and Epigenetics Proteomics Unit Stuebeweg 51 79108 Freiburg, Germany
contact emailmittler@ie-freiburg.mpg.de
lab head
Gerhard Mittler
contact affiliationProteomics Unit, Max Planck Institute of Immunobiology and Epigenetics
contact emailmittler@ie-freiburg.mpg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034457
PRIDE project URI
Repository Record List
[ + ]