PXD034453

PXD034453 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of fly brain cytoplasmic RNP granules containing the lncRNA mimi
Description	In neurons, mRNAs and associated RNA-binding proteins assemble into ribonucleoprotein (RNP) granules essential to regulate mRNA trafficking, local translation, and turnover. Dysregulation of RNA-protein condensation can disturb synaptic plasticity. We report that the novel lncRNA mimi is a constitutive and essential component of large cytoplasmic condensates (RNP granules) in fly neurons that are biochemically enriched by differential centrifugation. Here, employing relative iBAQ quantification we carry out a differential proteomic analysis of cytoplasmic RNP granules in wild-type versus delta-mimi mutant fly brains. Brain lysates from wild-type and mutant flies serve as a general proteome input control.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:33:06.819.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerhard Mittler
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-10 02:32:40	ID requested
1	2022-10-12 07:59:42	announced
⏵ 2	2023-11-14 08:33:09	announced	2023-11-14: Updated project metadata.

Grzejda D, Mach J, Schweizer JA, Hummel B, Rezansoff AM, Eggenhofer F, Panhale A, Lalioti ME, Cabezas Wallscheid N, Backofen R, Felsenberg J, Hilgers V, scaffolds neuronal granules to maintain nervous system maturity. Sci Adv, 8(39):eabo5578(2022) [pubmed]

submitter keyword: RNP granules

fly brain

neurons

lncRNA mimi;, FNE

RBP9, Drosophila

relative iBAQ

quantitative proteomics;

Gerhard Mittler
contact affiliation	Max Planck Institute of Immunobiology and Epigenetics Proteomics Unit Stuebeweg 51 79108 Freiburg, Germany
contact email	mittler@ie-freiburg.mpg.de
lab head
Gerhard Mittler
contact affiliation	Proteomics Unit, Max Planck Institute of Immunobiology and Epigenetics
contact email	mittler@ie-freiburg.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034453

PRIDE project URI

• PRIDE
  1. PXD034453
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of fly brain cytoplasmic RNP granules containing the lncRNA mimi