PXD034451

PXD034451 is an original dataset announced via ProteomeXchange.

Title	Inducible synthetic protein degradation system for phenotype control
Description	Synthetic control of cell factory phenotypes is needed for redirecting resources from cell growth to production. To achieve that ClpXP proteasome was introduced to yeast Saccharomyces cerevisiae under control of TetON induction. Growth essential (in synthetic defined medium) metabolic enzymes Acc1, Aro1, Hom3 were targeted to the ClpXP proteasome by introducing short ssrA tags. Inducing the ClpXP proteasome with doxycycline during exponential growth phase ceased the growth.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:46:33.897.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Stein
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-10 02:30:06	ID requested
1	2023-05-10 13:20:20	announced
⏵ 2	2023-11-14 08:46:34	announced	2023-11-14: Updated project metadata.

Kakko N, Rantasalo A, Koponen T, Vidgren V, Kannisto M, Maiorova N, Nygren H, Mojzita D, Penttil, ä M, Jouhten P, . ACS Synth Biol, 12(4):1021-1033(2023) [pubmed]

submitter keyword: Saccharomyces cerevisiae,ClpXP proteasome

Paula Jouhten
contact affiliation	Aalto University, Espoo, Finland
contact email	paula.jouhten@aalto.fi
lab head
Frank Stein
contact affiliation	EMBL
contact email	frank.stein@embl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD034451

PRIDE project URI

• PRIDE
  1. PXD034451
     1. Label: PRIDE project
     2. Name: Inducible synthetic protein degradation system for phenotype control