PXD034446

PXD034446 is an original dataset announced via ProteomeXchange.

Title	Total protein abundances upon Mcm6 knockdown in the murine CH12 cells
Description	Chromosomal translocations result from joining of DNA double-strand breaks (DSBs) and frequently cause cancer. Yet, the steps linking DSB formation to DSB ligation remain undeciphered. We report that DNA replication timing (RT) directly regulates lymphomagenic Myc translocations during antibody maturation in B-cells downstream of DSBs and independently of DSB frequency. Depletion of minichromosome-maintenance (MCM) complexes alters replication origin activity, decreases translocations and abrogates global RT. Ablating a single origin at Myc causes an early-to-late RT switch, loss of translocations and reduced nuclear proximity with a translocation partner locus, phenotypes that were reversed by restoring early RT. Disruption of shared early RT also reduced tumorigenic translocations in human leukemic cells. Thus, RT constitutes a new, unprecedented mechanism in translocation biogenesis linking DSB formation to DSB ligation
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:57:07.990.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 15:20:08	ID requested
1	2022-09-13 05:36:40	announced
⏵ 2	2023-11-14 08:57:08	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: murine CH12 cells,MCM6 knockdown, protein abundance

Rushad Rusi Pavri
contact affiliation	Research Institute of Molecular Pathology (IMP), Campus-Vienna-Biocenter-1, Vienna Biocenter, 1030 Vienna, Austria
contact email	rushad.pavri@imp.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034446
     1. Label: PRIDE project
     2. Name: Total protein abundances upon Mcm6 knockdown in the murine CH12 cells