PXD034445

PXD034445 is an original dataset announced via ProteomeXchange.

Title	HeLa cells were transfected with MARVELD1, then treated with or without HU treatment,following with IP and LC-MSMS analysis
Description	To analyze MARVELD1 interacting proteins, HeLa cells were transfected with MARVELD1-Flag or pcDNA3.1 (PC), then treated with 8 mM Hydroxyurea (MCE, USA) or DMSO for 24 h, and subjected to IP assays with anti-DYKDDDDK magnetic agarose (Thermo, USA). After washing five times with PBS buffer, samples were boiled in 2× SDS loading buffer, resolved in SDS-PAGE, visualized by Coomassie Blue staining, and subjected to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis (PTM Bio, China, project number: FA203GI).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:45:51.927.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Haoxiu Sun
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 15:19:47	ID requested
1	2023-05-10 09:02:49	announced
⏵ 2	2023-11-14 08:46:00	announced	2023-11-14: Updated project metadata.

Sun H, Liu C, Han F, Lin X, Cao L, Liu C, Ji Q, Cui J, Yao Y, Wang B, Liao Y, Nie H, Zhang Y, Li Y, The regulation loop of MARVELD1 interacting with PARP1 in DNA damage response maintains genome stability and promotes therapy resistance of cancer cells. Cell Death Differ, 30(4):922-937(2023) [pubmed]

submitter keyword: HeLa cells LC-MSMS MARVELD1 HU

Haoxiu Sun
contact affiliation	School of Life Science and Technology, Harbin Institute of Technology, Harbin City,China
contact email	shx13904819533@163.com
lab head
Haoxiu Sun
contact affiliation	School of Life Science and Technology, Harbin Institute of Technology
contact email	shx13904819533@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/05/PXD034445

PRIDE project URI

• PRIDE
  1. PXD034445
     1. Label: PRIDE project
     2. Name: HeLa cells were transfected with MARVELD1, then treated with or without HU treatment,following with IP and LC-MSMS analysis