PXD034443

PXD034443 is an original dataset announced via ProteomeXchange.

Title	Chronic intermittent ethanol exposure selectively impairs behavioral flexibility in aged rats compared to adult rats and modifies protein and protein pathways related to Alzheimer's disease
Description	Repeated excessive alcohol consumption increases the risk of developing cognitive decline and dementia. Hazardous drinking among older adults further increases such vulnerabilities. In order to understand the molecular mechanisms underlying alcohol-induced cognitive deficits in older adults, we performed a chronic intermittent ethanol exposure paradigm (ethanol or water gavage every other day 10 times) in 8-week-old young adult and 70-week-old aged rats. While spatial memory retrieval ascertained by probe trials in the Morris water maze was not significantly different between ethanol-treated and water-treated rats in both age groups after the fifth and tenth gavages, behavioral flexibility was impaired in ethanol-treated rats than water-treated rats in the aged group but not in the young adult group. Further proteomic and phosphoproteomic analyses on their hippocampal tissues by tandem mass tag mass spectrometry revealed ethanol-treatment-associated proteomic and phosphoproteomic differences distinct to the aged rats, including the upregulations of Prkcd protein level, several of its phosphosites, and its kinase activity and the same aspects in Camk2a but downregulated, and were enriched in pathways involved in neurotransmission regulation, synaptic plasticity, neuronal apoptosis, and insulin receptor signaling. In conclusion, our behavioral and proteomic results added several candidate proteins and pathways potentially associated with alcohol-induced cognitive decline in aged adults.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:36:34.094.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034443
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Benjamin Madden
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	phosphorylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 15:18:55	ID requested
1	2023-03-10 20:02:16	announced
⏵ 2	2023-11-14 08:36:35	announced	2023-11-14: Updated project metadata.

10.6019/PXD034443;

Ho AM, Peyton MP, Scaletty SJ, Trapp S, Schreiber A, Madden BJ, Choi DS, Matthews DB, Chronic Intermittent Ethanol Exposure Alters Behavioral Flexibility in Aged Rats Compared to Adult Rats and Modifies Protein and Protein Pathways Related to Alzheimer's Disease. ACS Omega, 7(50):46260-46276(2022) [pubmed]

submitter keyword: proteomics, hippocampus,chronic ethanol intermittent exposure, spatial memory, rats, behavioral flexibility, water maze, phosphoproteomics

Ada Ho
contact affiliation	Mayo Clinic, Research Associate
contact email	Ho.Ada@mayo.edu
lab head
Benjamin Madden
contact affiliation	Mayo Clinic
contact email	madden.benjamin@mayo.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034443
     1. Label: PRIDE project
     2. Name: Chronic intermittent ethanol exposure selectively impairs behavioral flexibility in aged rats compared to adult rats and modifies protein and protein pathways related to Alzheimer's disease