PXD034430

PXD034430 is an original dataset announced via ProteomeXchange.

Title	Redox regulation of cytosolic translation in rice during fungal infection identified by quantitative proteomics
Description	Pathogen attack can increase plant levels of reactive oxygen species (ROS), which then act as signaling molecules activating plant defense. Elucidating these processes is crucial to understanding the redox signaling pathways in plant defense responses. By an iodo TMT–based quantitative proteomic approach, we mapped 4,292 oxidized cysteine sites in 2,808 proteins in rice leaves. Oxidized proteins were involved in gene expression, peptide biosynthetic processes, stress responses, ROS metabolic processes, and translation pathways. Magnaporthe oryzae infection led to increased oxidation modification levels of 531 cysteine sites in 454 proteins, including many transcriptional regulators and ribosomal proteins. Ribo-seq analysis revealed that the oxidation modification of ribosomal proteins promoted the translational efficiency of many mRNAs involved in defense response pathways, thereby affecting rice immunity. Our results suggest that increased oxidative modification of ribosomal proteins in rice leaves promotes cytosolic translation, revealing a novel function of post-translational modifications. Furthermore, Wthee identified oxidation-sensitive plant proteins, which identified here provided a valuable research resource of research on protein redox regulation and could guide future mechanistic studies. Our results suggest that increased oxidative modification of ribosomal proteins in rice leaves promotes cytosolic translation, revealing a novel function of post-translational modifications.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:48:34.130.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoyang Chen
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 14:25:25	ID requested
1	2023-06-06 01:16:09	announced
⏵ 2	2023-11-14 08:48:37	announced	2023-11-14: Updated project metadata.

Chen X, Xu Q, Yue Y, Duan Y, Liu H, Chen X, Huang J, Zheng L, Comparative oxidation proteomics analyses suggest redox regulation of cytosolic translation in rice leaves upon Magnaporthe oryzae infection. Plant Commun, 4(3):100550(2023) [pubmed]

submitter keyword: ROS

protein oxidation

translation

infection

immunity

rice

Xiaoyang Chen
contact affiliation	State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China
contact email	1149806052@qq.com
lab head
Xiaoyang Chen
contact affiliation	The Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China
contact email	1149806052@qq.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/06/PXD034430

PRIDE project URI

• PRIDE
  1. PXD034430
     1. Label: PRIDE project
     2. Name: Redox regulation of cytosolic translation in rice during fungal infection identified by quantitative proteomics