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PXD034414

PXD034414 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMembrane atg8ylation coordinates stress granule formation and mTOR inactivation in response to lysosomal damage
DescriptionWe report that lysosomal damage is a hitherto unknown inducer of stress granule (SG) formation and that the process termed membrane atg8ylation coordinates SG formation with mTOR inactivation during lysosomal stress. SGs were induced by lysosome-damaging agents including SARS-CoV-2ORF3a, Mycobacterium tuberculosis, and proteopathic tau. During damage, mammalian ATG8s directly interacted with the core SG proteins NUFIP2 and G3BP1. Atg8ylation was needed for their recruitment to damaged lysosomes independently of SG condensates whereupon NUFIP2 contributed to mTOR inactivation via the Ragulator-RagA/B complex. Thus, cells employ membrane atg8ylation to control and coordinate SG and mTOR responses to lysosomal damage.
HostingRepositoryMassIVE
AnnounceDate2023-01-26
AnnouncementXMLSubmission_2023-01-26_08:09:59.394.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrett Phinney
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-09 12:36:06ID requested
12023-01-26 08:10:00announced
Publication List
Jia J, Wang F, Bhujabal Z, Peters R, Mudd M, Duque T, Allers L, Javed R, Salemi M, Behrends C, Phinney B, Johansen T, Deretic V, Stress granules and mTOR are regulated by membrane atg8ylation during lysosomal damage. J Cell Biol, 221(11):(2022) [pubmed]
Keyword List
submitter keyword: mTOR, lysosomal damage
Contact List
Vojo Deretic
contact affiliationDepartment of Molecular Genetics and Microbiology University of New Mexico Health Sciences Center
contact emailvderetic@salud.unm.edu
lab head
Brett Phinney
contact affiliationUC Davis
contact emailbrettsp@ucdavis.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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