PXD034413

PXD034413 is an original dataset announced via ProteomeXchange.

Title	Spatially-targeted optical microproteomics timecourse of rat myocardial infarction
Description	Myocardial infarction (MI) results from occlusion of blood supply to the heart muscle causing death of cardiac muscle cells. Following myocardial infarction (MI), scar formation acts to mechanically stabilise the injured heart to prevent rupture and death. While fibroblasts and macrophages are implicated in post-MI scar formation and maturation, their exact contributions to this process are poorly characterised, especially in the long-term (i.e., beyond 1-week post-MI). Here, we employ state-of-the-art spatially-targetted optical micro-proteomics (STOMP) to isolate proteomes of tissue fractions enriched for fibroblasts (sma+) and macrophages (cd68+) over a 6-week post-MI timecourse and compare them to whole-scar proteome. We illustrate dynamic, specific changes in sma- and cd68-enriched fraction protein composition over 1-6 weeks post-MI with some of these changes reflected in whole-infarct preparations. These results link specific cell populations to specific protein changes in whole infarct.
HostingRepository	PRIDE
AnnounceDate	2023-10-31
AnnouncementXML	Submission_2023-10-31_10:31:58.378.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	VenkateshMallikarjun
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	dehydroalanine (Cys); acetylated residue; monohydroxylated residue; L-cysteic acid (L-cysteine sulfonic acid); iodoacetic acid derivatized residue; deamidated residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 08:34:59	ID requested
⏵ 1	2023-10-31 10:31:58	announced

Dataset with its publication pending

submitter keyword: cardiac, heart, macrophage, cd68,rat, sma, microproteomics, fibroblast, myocardial infarction

SarahEwald
contact affiliation	Assistant Professor of Microbiology, Immunology, and Cancer Biology Associate Director for Diversity, Equity, and Inclusio at the Carter Immunology Center University of Virginia Charlottesville, VA
contact email	e2s@virginia.edu
lab head
VenkateshMallikarjun
contact affiliation	University of Manchester
contact email	vm9uq@virginia.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/10/PXD034413

PRIDE project URI

• PRIDE
  1. PXD034413
     1. Label: PRIDE project
     2. Name: Spatially-targeted optical microproteomics timecourse of rat myocardial infarction