PXD034411

PXD034411 is an original dataset announced via ProteomeXchange.

Title	Peroxiredoxins act as a post-translational modification in cytoplasmic protein quality control
Description	The redox state of proteins is essential for their function and to guarantee cell fitness. In the endoplasmic reticulum, cells recognize and resolve undesirable cysteine oxidations by protein quality control machinery that involves protein disulfide isomerases. While disulfide bonds are uncommon in the reducing environment of the cytoplasm, they play a key role in redox signaling during oxidative stress and disease states. Using global proteomic analyses, here we show that the cytoplasmic peroxiredoxin Tsa1 covalently binds cysteine residues of hundreds of proteins, including metabolic enzymes, and acts as a protective chaperone to maintain protein function. Thioredoxins maintain the balance of this peroxiredoxin post-translational mark. Our study unveils a mechanism involving the peroxiredoxin-thioredoxin cycle in cytoplasmic protein quality control in eukaryotes
HostingRepository	PRIDE
AnnounceDate	2025-01-27
AnnouncementXML	Submission_2025-01-27_00:28:47.088.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Eva Borràs
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	L-cysteine methyl disulfide; N-ethylmaleimide derivatized cysteine; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-09 08:17:27	ID requested
⏵ 1	2025-01-27 00:28:47	announced

10.1016/J.CELREP.2024.115224;

submitter keyword: peroxiredoxin, mass spectrometry

Eduard Sabido
contact affiliation	CRG, UPF
contact email	eduard.sabido@crg.eu
lab head
Eva Borràs
contact affiliation	UPF
contact email	eva.borras@upf.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034411
     1. Label: PRIDE project
     2. Name: Peroxiredoxins act as a post-translational modification in cytoplasmic protein quality control