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PXD034402

PXD034402 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDigestion time course of human liver plasma membrane
DescriptionThe selection of signature peptides is critical for reliable peptide quantification. The purpose of this study was to comprehensively assess the digestion efficiency and stability of tryptic peptides and to identify optimal signature peptides for human hepatic transporters and membrane marker proteins. The plasma membrane fraction of the human liver was digested at different time points (0.5, 1, 2, 4, 8, 16, 24, and 48 h) and the peptides were comprehensively quantified using quantitative proteomics.
HostingRepositoryjPOST
AnnounceDate2022-10-12
AnnouncementXMLSubmission_2022-10-11_08:00:04.281.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSumio Ohtsuki
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListS-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-08 01:39:44ID requested
12022-10-11 08:00:05announced
Publication List
Mori A, Masuda T, Ito S, Ohtsuki S, Human Hepatic Transporter Signature Peptides for Quantitative Targeted Absolute Proteomics: Selection, Digestion Efficiency, and Peptide Stability. Pharm Res, 39(11):2965-2978(2022) [pubmed]
Keyword List
submitter keyword: human, liver, SWATH, DIA
Contact List
Sumio Ohtsuki
lab head
Sumio Ohtsuki
contact affiliationKumamoto University
dataset submitter
Full Dataset Link List
jPOST dataset URI
Dataset FTP location
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