PXD034398

PXD034398 is an original dataset announced via ProteomeXchange.

Title	LncRNA EPR-induced METTL7A1 modulates target gene translation
Description	EPR is a long non-coding RNA (lncRNA) that controls cell proliferation in mammary gland cells by regulating gene transcription. Here, we report on Mettl7a1 as a direct target of EPR. We show that EPR induces Mettl7a1 transcription by rewiring three-dimensional chromatin interactions at the Mettl7a1 locus. Our data indicate that METTL7A1 contributes to EPR-dependent inhibition of TGF-β signaling. METTL7A1 is absent in tumorigenic murine mammary gland cells and its human ortholog (METTL7A) is downregulated in breast cancers. Importantly, re-expression of METTL7A1 in 4T1 tumorigenic cells attenuates their transformation potential, with the putative methyltransferase activity of METTL7A1 being dispensable for its biological functions. We found that METTL7A1 localizes in the cytoplasm whereby it interacts with factors implicated in the early steps of mRNA translation, associates with ribosomes, and affects the levels of target proteins without altering mRNA abundance. Overall, our data indicates that METTL7A1 —a transcriptional target of EPR— modulates translation of select transcripts.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-11_16:08:26.513.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034398
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Roberto Gherzi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-07 01:19:32	ID requested
⏵ 1	2022-08-11 16:08:27	announced

Briata P, Caputo L, Zapparoli E, Marcaccini E, Passalacqua M, Brondolo L, Bordo D, Rossi A, Nicoletti C, Bucci G, Puri PL, Inga A, Gherzi R, LncRNA EPR-induced METTL7A1 modulates target gene translation. Nucleic Acids Res, 50(13):7608-7622(2022) [pubmed]

submitter keyword: lncERNA, translation, RNA binding protein

Roberto Gherzi
contact affiliation	Gene Expression Regulation Laboratory, IRCCS Ospedale Policlinico San Martino, 16132 Genova, Italy
contact email	roberto.gherzi@hsanmartino.it
lab head
Roberto Gherzi
contact affiliation	Ospedale Policlinico San Martino IRCCS
contact email	roberto.gherzi@hsanmartino.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034398
     1. Label: PRIDE project
     2. Name: LncRNA EPR-induced METTL7A1 modulates target gene translation