PXD034396

PXD034396 is an original dataset announced via ProteomeXchange.

Title	Evaluation of the potential risk of Easy-nLC 1200 in phosphoproteomics
Description	Liquid chromatography-mass spectrometry (LC-MS) is a major tool for large scale qualitative and/or quantitative analysis of protein phosphorylation in cells or tissues. Performance of LC is pivotal for the success of phosphoprotemics in both sensitivity and reproducibility. Here we report that the widely used Easy-nLC 1200 has poor performance in analyzing phosphopeptides, particularly in terms of sensitivity and reproducibility, whereas its predecessor, Easy-nLC 1000 has much better performance.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:18:35.367.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yuanya Zhang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-07 01:18:23	ID requested
1	2023-03-10 15:41:50	announced
⏵ 2	2023-11-14 08:18:36	announced	2023-11-14: Updated project metadata.

Duan X, Zhang Y, Huang X, Ma X, Gao H, Wang Y, Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers. ACS Omega, 7(51):47806-47811(2022) [pubmed]

submitter keyword: phosphoproteomics,LC-MS,phosphorylation

Yingchun Wang
contact affiliation	State Key Laboratory of Molecular Developmental Biology, Institute of Genetics andDevelopmental Biology, Chinese Academy of Sciences
contact email	yyazhang@genetics.ac.cn
lab head
Yuanya Zhang
contact affiliation	Institute of Genetics and Developmental Biology
contact email	yyazhang@genetics.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD034396

PRIDE project URI

• PRIDE
  1. PXD034396
     1. Label: PRIDE project
     2. Name: Evaluation of the potential risk of Easy-nLC 1200 in phosphoproteomics