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PXD034391

PXD034391 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleHuman sepsis with plasma samples
DescriptionThe project examines the mechanisms of neutrophil dysfunction during sepsis. Our work uncovered the central role of cell free circulating histones in eliminating mature neutrophil in favour of immature cells and characterized the mechanisms that regulate their release following systemic infection. Mature and immature neutrophil Ly6Ghigh and Ly6Glow populations isolated from the spleens of WT and TCRα-deficient mice either naïve or infected with C. albicans were characterized. In addition, these populations were compared to neutrophils isolated from WT mice receiving Clodronate-liposomes and recombinant G-CSF. These studies demonstrated that T-cell derived histones drive the release of G-CSF in the spleen and progressively eliminate mature neutrophils by shortening their lifespan. Finally, we conducted proteomic analysis of plasmas isolated from patients with microbial sepsis to correlate markers of neutrophil death to plasma cytokine and histone levels, confirming the pathogenic role these molecules play during sepsis in humans.
HostingRepositoryPRIDE
AnnounceDate2022-07-01
AnnouncementXMLSubmission_2022-07-01_09:29:58.964.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSpyros Vernardis
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-07 01:09:06ID requested
12022-07-01 09:29:59announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: human plasma sepsis
Contact List
Markus Ralser
contact affiliationMarkus Ralser, Prof. Dr. Einstein Professor of Biochemistry Head, Institute of Biochemistry, Charité University Medicine, Berlin markus.ralser@charite.de Senior Group Leader, The Francis Crick Institute, London markus.ralser@crick.ac.uk
contact emailmarkus.ralser@crick.ac.uk
lab head
Spyros Vernardis
contact affiliationThe Francis Crick Institute
contact emailspyros.vernardis@crick.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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