PXD034391 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human sepsis with plasma samples |
Description | The project examines the mechanisms of neutrophil dysfunction during sepsis. Our work uncovered the central role of cell free circulating histones in eliminating mature neutrophil in favour of immature cells and characterized the mechanisms that regulate their release following systemic infection. Mature and immature neutrophil Ly6Ghigh and Ly6Glow populations isolated from the spleens of WT and TCRα-deficient mice either naïve or infected with C. albicans were characterized. In addition, these populations were compared to neutrophils isolated from WT mice receiving Clodronate-liposomes and recombinant G-CSF. These studies demonstrated that T-cell derived histones drive the release of G-CSF in the spleen and progressively eliminate mature neutrophils by shortening their lifespan. Finally, we conducted proteomic analysis of plasmas isolated from patients with microbial sepsis to correlate markers of neutrophil death to plasma cytokine and histone levels, confirming the pathogenic role these molecules play during sepsis in humans. |
HostingRepository | PRIDE |
AnnounceDate | 2022-07-01 |
AnnouncementXML | Submission_2022-07-01_09:29:58.964.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Spyros Vernardis |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-07 01:09:06 | ID requested | |
⏵ 1 | 2022-07-01 09:29:59 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: human plasma sepsis |
Contact List
Markus Ralser |
contact affiliation | Markus Ralser, Prof. Dr. Einstein Professor of Biochemistry Head, Institute of Biochemistry, Charité University Medicine, Berlin markus.ralser@charite.de Senior Group Leader, The Francis Crick Institute, London markus.ralser@crick.ac.uk |
contact email | markus.ralser@crick.ac.uk |
lab head | |
Spyros Vernardis |
contact affiliation | The Francis Crick Institute |
contact email | spyros.vernardis@crick.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD034391 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034391
- Label: PRIDE project
- Name: Human sepsis with plasma samples