To characterize the changes of phosphorylation signaling cascades induced by LAT1 inhibition in MIA PaCa-2 cells treated with LAT1 specific inhibitor JPH203, immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometry with MS2-based quantitation of tandem mass tag were utilized. To characterize the changes of protein expressions induced by LAT1 inhibition and AKT inhibition in MIA PaCa-2 cells exposed to LAT1 specific inhibitor JPH203 with or without AKT inhibitor MK-2206, immobilized metal affinity chromatography and liquid chromatography-tandem mass spectrometry with MS2-based quantitation of tandem mass tag were utilized.