PXD034376

PXD034376 is an original dataset announced via ProteomeXchange.

Title	Phagosome proteome of mock and Atg8 silenced Entamoeba histolytica strains
Description	The enteric protozoan parasite Entamoeba histolytica have high phagocytic ability. Phagocytosis is also important for the pathogenicity of this parasite; molecular mechanisms of phagocytosis and phagosome maturation is focused. Atg8 is well studied autophagy marker protein. We previously shown that E. histolytica Atg8 translocate to nascent trogosomes and expression silencing of the Atg8 caused retardation of phagosome acidification. To investigate how Atg8 regulates phagosome maturation, here we conducted proteome analysis of phagosomes isolated from an E. histolytica strain in which atg8 gene expression are silenced (atg8 gene silenced, atg8-gs) and its mock control strain (transfected with psAP2-Gunma).
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:05.572.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034376
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kumiko Tsukui
SpeciesList	scientific name: Entamoeba histolytica HM-1:IMSS-A; NCBI TaxID: 885318;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion ETD

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-06 07:19:57	ID requested
1	2022-10-20 01:06:57	announced
⏵ 2	2023-11-14 08:56:06	announced	2023-11-14: Updated project metadata.

10.6019/PXD034376;

submitter keyword: autophagy, lysosome, Atg8,Entamoeba histolytica, serum coated beads, phagosome

Kumiko Nakada-Tsukui
contact affiliation	Department of Parasitology, National Institute of Infectious Diseases
contact email	kumiko@niid.go.jp
lab head
Kumiko Tsukui
contact affiliation	National Institute of Infectious Diseases
contact email	kumiko@niid.go.jp
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034376
     1. Label: PRIDE project
     2. Name: Phagosome proteome of mock and Atg8 silenced Entamoeba histolytica strains