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PXD034370 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMicroscopy-based functional single cell proteomic profiling of cells with abnormal DNA damage response dynamics
DescriptionTumor heterogeneity is an important source of cancer therapy resistance. Single cell proteomics has the potential to decipher protein content leading to heterogeneous cellular phenotypes. Single-Cell ProtEomics by Mass Spectrometry (SCoPE-MS) is a recently developed, promising, unbiased proteomic profiling techniques, which allows profiling several tens of single cells for >1000 proteins per cell. However, a method to link single cell proteomes with cellular behaviors is needed to advance this type of profiling technique. Here, we developed a microscopy-based functional single cell proteomic profiling technology, called FUNpro, to link the proteome of individual cells with phenotypes of interest, even if the phenotypes are dynamic or the cells of interest are sparse. FUNpro enables one i) to screen thousands of cells with subcellular resolution and monitor (intra)cellular dynamics using a custom-built microscope, ii) to real-time analyze (intra)cellular dynamics of individual cells using an integrated cell tracking algorithm, iii) to promptly isolate the cells displaying phenotypes of interest, and iv) to single cell proteomically profile the isolated cells. We applied FUNpro to proteomically profile a newly identified small subpopulation of U2OS osteosarcoma cells displaying an abnormal, prolonged DNA damage response (DDR) after ionizing radiation (IR). With this, we identified the PDS5A protein contributing to the abnormal DDR dynamics and helping the cells survive after IR.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterPin-Rui Su
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListdeamidated residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-06 06:38:31ID requested
12022-07-07 06:24:05announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: single-cell proteomics
Contact List
Miao-Ping Chien
contact affiliationDepartment of Molecular Genetics, Erasmus University Medical Center, Rotterdam, The Netherlands. Erasmus MC Cancer Institute, The Netherlands. Oncode Institute, Utrecht, The Netherlands.
contact emailm.p.chien@erasmusmc.nl
lab head
Pin-Rui Su
contact affiliationErasmus MC
contact emailp.su@erasmusmc.nl
dataset submitter
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Dataset FTP location
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