PXD034369

PXD034369 is an original dataset announced via ProteomeXchange.

Title	Isolation and Characterisation of EVs from Feline Plasma: A Comparative Analysis with Human Plasma Derived EVs
Description	Extracellular vesicles (EVs) are nanoparticles found in all biological fluids, capable of transporting biological material around the body. Extensive research into the physiological role of EVs has led to the development of the Minimal Information for Studies of Extracellular Vesicles (MISEV) framework in 2018. This framework guides the standardisation of protocols in the EV field. To date, the focus has been on EVs of human origin. As comparative medicine progresses, there has been a drive to study similarities between diseases in humans and animals. To successfully research EVs in felines, we must validate the application of the MISEV guidelines in this group. EVs were isolated from the plasma of healthy humans and felines. EV characterisation was carried out according to the MISEV guidelines. Human and feline plasma showed a similar concentration of EVs, comparable expression of known EV markers and analogous particle to protein ratios. Mass spectrometry analyses showed that the proteomic signature of EVs from humans and felines were similar. Asymmetric field flow fractionation showed two distinct subpopulations of EVs isolated from human plasma, whereas only one subpopulation was isolated from feline plasma and metabolomic profiling showed similar profiles for humans and felines. In conclusion, isolation, and characterisation of EVs from humans and felines show that MISEV2018 guidelines may also be applied to felines. Potential comparative medicine studies of EVs may provide a model for studying naturally occurring diseases in both species.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:35.616.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kieran Wynne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-06 06:38:07	ID requested
1	2022-07-13 09:00:01	announced
⏵ 2	2023-11-14 08:56:36	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Human,Extracellular vesicles, Cat, Bruker timsTOF Pro

Amanda McCann
contact affiliation	University College Dublin, School of Medicine, Conway Institute Belfield Dublin 4, Ireland.
contact email	amanda.mccann@ucd.ie
lab head
Kieran Wynne
contact affiliation	University College Dublin
contact email	kieran.wynne1@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD034369

PRIDE project URI

• PRIDE
  1. PXD034369
     1. Label: PRIDE project
     2. Name: Isolation and Characterisation of EVs from Feline Plasma: A Comparative Analysis with Human Plasma Derived EVs