PXD034360

PXD034360 is an original dataset announced via ProteomeXchange.

Title	Targeted proteomic analysis of small GTPases in radioresistant breast cancer cells
Description	Radiotherapy benefits more than 50% of cancer patients and cures 40% of them, where ionizing radiation deposits energy to cells and tissues, thereby eliciting DNA damage and resulting in cell death. Small GTPases are a superfamily of proteins that play critical roles in cell signaling. Several small GTPases, including RAC1, RHOB and RALA, were previously shown to modulate radioresistance in cancer cells. However, there is no systematic proteomic study on small GTPases that regulate radioresistance in cancer cells. Herein, we applied a high-throughput scheduled multiple-reaction monitoring (MRM) method, along with the use of synthetic stable isotope-labeled peptides, to identify differentially expressed small GTPase proteins in two pairs of breast cancer cell lines, MDA-MB-231 and MCF7, and their corresponding radioresistant lines. We identified 7 commonly altered small GTPase proteins with over 1.5-fold change in the two pairs of cell lines. We also discovered ARFRP1 as a novel radioresistance regulator, where its downregulation promotes radioresistance in breast cancer cells. Together, this represents the first comprehensive assessment about the differential expression of the small GTPase proteome associated with the development of radioresistance in breast cancer cells. Our work also uncovered ARFRP1 as a new target for enhancing radiation sensitivity in breast cancer.
HostingRepository	PRIDE
AnnounceDate	2024-01-26
AnnouncementXML	Submission_2024-01-26_05:44:49.169.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zi Gao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	TSQ Altis

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-06 03:08:01	ID requested
⏵ 1	2024-01-26 05:44:50	announced

10.1021/acs.analchem.2c02389;

Gao Z, Yang YY, Huang M, Qi TF, Wang H, Wang Y, Targeted Proteomic Analysis of Small GTPases in Radioresistant Breast Cancer Cells. Anal Chem, 94(43):14925-14930(2022) [pubmed]

submitter keyword: breast cancer, radioresistance, radiation therapy, MRM,targeted proteomics, small GTPase

Yinsheng Wang
contact affiliation	Distinguished Professor Department of Chemistry University of California Riverside
contact email	yinsheng@ucr.edu
lab head
Zi Gao
contact affiliation	University of California, Riverside
contact email	zgao015@ucr.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034360
     1. Label: PRIDE project
     2. Name: Targeted proteomic analysis of small GTPases in radioresistant breast cancer cells