PXD034357

PXD034357 is an original dataset announced via ProteomeXchange.

Title	Endometrial gland impacts on stromal cell decidualization
Description	Uterine glands and, by inference, their secretions impact uterine receptivity, blastocyst implantation, stromal cell decidualization, and placental development. Changes in gland function across the menstrual cycle are impacted by steroid hormones, estrogen and progesterone, as well as stroma-derived factors. Using an endometrial epithelial organoid (EEO) system, transcriptome and proteome analyses identified distinct responses of the EEO to steroid hormones and prostaglandin E2 (PGE2). Notably, steroid hormones and PGE2 modulated the basolateral secretion of EEO proteins, where cystatin C (CST3) was significantly increased by progesterone and PGE2. CST3 treatment of decidualizing stromal cells significantly decreased the decidualization markers PRL and IGFBP1. The attenuation of stromal cell decidualization via CST3 suggests a role for uterine gland-derived proteins in controlling the extent of decidualization. These findings provide evidence that uterine gland-derived factors directly impact stromal cell decidualization, which has strong implications for better understanding pregnancy establishment and female fertility in humans.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:05:50.455.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Brian Mooney
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-06 03:04:21	ID requested
1	2023-10-11 15:13:01	announced
⏵ 2	2023-11-14 09:05:56	announced	2023-11-14: Updated project metadata.

Fitzgerald HC, Kelleher AM, Ranjit C, Schust DJ, Spencer TE, Basolateral secretions of human endometrial epithelial organoids impact stromal cell decidualization. Mol Hum Reprod, 29(4):(2023) [pubmed]

submitter keyword: hormone,endometrium, decidualization, gland, PGE2, stroma

Thomas E Spencer
contact affiliation	Curators’ Distinguished Professor of Animal Sciences and Obstetrics, Gynecology and Women’s Health, University of Missouri, Columbia
contact email	spencerte@missouri.edu
lab head
Brian Mooney
contact affiliation	University of Missouri, Columbia
contact email	mooneyb@missouri.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034357
     1. Label: PRIDE project
     2. Name: Endometrial gland impacts on stromal cell decidualization