PXD034356 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A slit-diaphragm-associated protein network for dynamic control of renal filtration |
Description | The filtration of blood in the kidney which is crucial for mammalian life is determined by the slit-diaphragm (SD), a proteinaceous junction between the foot processes of renal podocytes. Based on studies of structure and function the SD has been hypothesized to operate as a final barrier or as a ‘molecular sensor’ of renal filtration. Using high-resolution proteomic analysis of SDs affinity-isolated from rodent kidney, we show that the native SD is built from the known junction-forming components Nephrin, Neph1 and Podocin and a co-assembled high-molecular weight network of proteins. The network constituents cover distinct classes of proteins including signaling receptors, kinases and phosphatases, transporters and scaffolds. Knock-out or knock-down of either the core components or the selected network constituents tyrosine kinase MER (MERTK), atrial natriuretic peptide receptor C (ANPRC) and protein ITM2B resulted in target-specific impairment or disruption of the filtration process. Thus, our results identify the SD as a multi-component system that is endowed with context-dependent dynamics via a co-assembled protein network. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-20 |
AnnouncementXML | Submission_2022-09-20_07:44:09.745.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD034356 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Alexander Haupt |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | S-carboxamidoethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive; LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-06 02:35:46 | ID requested | |
⏵ 1 | 2022-09-20 07:44:10 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: kidney, renal filtration, slit-diaphragm, podocytes, mouse, rat, Nephrin, Podocin, Neph1, LC-MS/MS, AP-MS, Orbitrap |
Contact List
Prof. Dr. Bernd Fakler |
contact affiliation | Institute of Physiology II Universität Freiburg Germany |
contact email | bernd.fakler@physiologie.uni-freiburg.de |
lab head | |
Alexander Haupt |
contact affiliation | University of Freiburg, Germany |
contact email | alexander.haupt@physiologie.uni-freiburg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD034356 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034356
- Label: PRIDE project
- Name: A slit-diaphragm-associated protein network for dynamic control of renal filtration