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PXD034356

PXD034356 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA slit-diaphragm-associated protein network for dynamic control of renal filtration
DescriptionThe filtration of blood in the kidney which is crucial for mammalian life is determined by the slit-diaphragm (SD), a proteinaceous junction between the foot processes of renal podocytes. Based on studies of structure and function the SD has been hypothesized to operate as a final barrier or as a ‘molecular sensor’ of renal filtration. Using high-resolution proteomic analysis of SDs affinity-isolated from rodent kidney, we show that the native SD is built from the known junction-forming components Nephrin, Neph1 and Podocin and a co-assembled high-molecular weight network of proteins. The network constituents cover distinct classes of proteins including signaling receptors, kinases and phosphatases, transporters and scaffolds. Knock-out or knock-down of either the core components or the selected network constituents tyrosine kinase MER (MERTK), atrial natriuretic peptide receptor C (ANPRC) and protein ITM2B resulted in target-specific impairment or disruption of the filtration process. Thus, our results identify the SD as a multi-component system that is endowed with context-dependent dynamics via a co-assembled protein network.
HostingRepositoryPRIDE
AnnounceDate2022-09-20
AnnouncementXMLSubmission_2022-09-20_07:44:09.745.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD034356
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexander Haupt
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListS-carboxamidoethyl-L-cysteine; 2-pyrrolidone-5-carboxylic acid (Glu); 2-pyrrolidone-5-carboxylic acid (Gln); phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive; LTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-06 02:35:46ID requested
12022-09-20 07:44:10announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: kidney, renal filtration, slit-diaphragm, podocytes, mouse, rat, Nephrin, Podocin, Neph1, LC-MS/MS, AP-MS, Orbitrap
Contact List
Prof. Dr. Bernd Fakler
contact affiliationInstitute of Physiology II Universität Freiburg Germany
contact emailbernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliationUniversity of Freiburg, Germany
contact emailalexander.haupt@physiologie.uni-freiburg.de
dataset submitter
Full Dataset Link List
Dataset FTP location
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