PXD034347

PXD034347 is an original dataset announced via ProteomeXchange.

Title	MPK4 interacting proteins in guard cells through AP-MS
Description	Plants are often challenged by numerous biotic and abiotic environmental stresses. They have evolved efficient signaling networks in response to the environmental challenges, such as pathogen invasion, drought, CO2 changes. Guard cells are specialized epidermal cells enclosing pores on leaf surface, aka, stomata. Stomatal guard cells are at the frontline of biotic and abiotic stress responses. The opening and closing of stomata are regulated by a sophisticated intracellular signaling networks. Mitogen-activated protein kinase 4 (MPK4) was first identified as negative regulator in systemic acquired resistance (SAR). It was also play important roles in cytokinesis, reproduction, and photosynthesis. MPK4 has higher expression levels in plant guard cells than in mesophyll cells. Arabidopsis mpk4 mutant has higher levels of salicylic acid (SA) and reactive oxygen species (ROS), which are related with stomatal movement. The specific function of MPK4 in guard cells is unknown. In order to understand MPK4 functions in guard cells, it is essential to investigate MPK4-associated protein complex. Here we focus on identification of guard cell specific MPK4 complex using affinity purification following by mass spectrometry (AP-MS). Potential substrates were selected and validated with yeast two hybrid (Y2H).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:37:51.576.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chuwei Lin
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-06 02:11:56	ID requested
1	2024-04-30 06:47:49	announced
⏵ 2	2024-10-22 06:37:52	announced	2024-10-22: Updated project metadata.

10.1016/j.jprot.2023.104903;

Lin C, Dai D, Zhao G, Joe S, Chen S, Identification of MPK4 interacting proteins in guard cells. J Proteomics, 281():104903(2023) [pubmed]

submitter keyword: AP-MS, Arabidopsis thaliana,MPK4, guard cells, single-cell-type proteomics

Sixue Chen
contact affiliation	Department of Biology, University of Florida, Gainesville, FL, USA Proteomics and Mass Spectrometry, Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida, Gainesville, FL, USA
contact email	schen@ufl.edu
lab head
Chuwei Lin
contact affiliation	University of Florida
contact email	chuwei.lin@ufl.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/04/PXD034347

PRIDE project URI

• PRIDE
  1. PXD034347
     1. Label: PRIDE project
     2. Name: MPK4 interacting proteins in guard cells through AP-MS