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PXD034262

PXD034262 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIntegrative Proteome and Phosphoproteome Profiling of Early Cold Response in Maize Seedlings
DescriptionCold limits the growth and yield of maize in temperate regions, but the molecular mechanism of cold adpation remains largely unexplored in maize. To identify early molecular events during cold shock, maize seedlings were treated under 4℃ for 30 minutes and 2 hours, and analyzed at both the proteome and phosphoproteome levels. Over 8,500 proteins and 19,300 phosphopeptides were quantified. About 660 and 620 proteins were cold-responsive at protein abundance or site-specific phosphorylation levels, but only 65 proteins were shared between them. Functional enrichment analysis of cold responsive proteins and phosphoproteins revealed that early cold response in maize is associated with photosynthesis-light reaction, spliceosome, endocytosis, and defense response, consistent with similar studies in Arabidopsis. Thirty-two photosynthesis proteins were down-regulated at protein levels, and 48 spliceosome proteins were altered at site-specific phosphorylation levels. Thirty-one kinases and 33 transcriptional factors were cold-responsive at protein, phosphopeptides, or site-specific phosphorylation levels. Our results showed that maize seedlings respond to cold shock rapidly, at both the proteome and phosphoproteome levels. This study provides a comprehensive landscape at the cold-responsive proteome and phosphoproteome in maize seedlings that can be a significant resource to understand how C4 plants respond to a sudden temperature drop.
HostingRepositoryiProX
AnnounceDate2022-06-01
AnnouncementXMLSubmission_2022-09-28_23:49:22.746.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterzhiping Deng
SpeciesList scientific name: Zea mays; NCBI TaxID: 4577;
ModificationListiodoacetamide derivatized residue; phosphorylated residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-06-01 18:36:42ID requested
12022-06-01 18:36:57announced
22022-09-28 23:49:23announced2022-09-29: Update publication information.
Publication List
Xing J, Tan J, Feng H, Zhou Z, Deng M, Luo H, Deng Z, Integrative Proteome and Phosphoproteome Profiling of Early Cold Response in Maize Seedlings. Int J Mol Sci, 23(12):(2022) [pubmed]
Keyword List
submitter keyword: maize, cold stress, TMT-labeling, proteome, phosphoproteome, seedlings, photosynthesis, spliceosome
Contact List
Zhiping Deng
contact affiliationZhejiang Academy of Agricultural Sciences
contact emailzhipingdeng@126.com
lab head
zhiping Deng
contact affiliationZhejiang Academy of Agricultural Sciences
contact emailzhipingdeng@126.com
dataset submitter
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iProX dataset URI