PXD034262

PXD034262 is an original dataset announced via ProteomeXchange.

Title	Integrative Proteome and Phosphoproteome Profiling of Early Cold Response in Maize Seedlings
Description	Cold limits the growth and yield of maize in temperate regions, but the molecular mechanism of cold adpation remains largely unexplored in maize. To identify early molecular events during cold shock, maize seedlings were treated under 4℃ for 30 minutes and 2 hours, and analyzed at both the proteome and phosphoproteome levels. Over 8,500 proteins and 19,300 phosphopeptides were quantified. About 660 and 620 proteins were cold-responsive at protein abundance or site-specific phosphorylation levels, but only 65 proteins were shared between them. Functional enrichment analysis of cold responsive proteins and phosphoproteins revealed that early cold response in maize is associated with photosynthesis-light reaction, spliceosome, endocytosis, and defense response, consistent with similar studies in Arabidopsis. Thirty-two photosynthesis proteins were down-regulated at protein levels, and 48 spliceosome proteins were altered at site-specific phosphorylation levels. Thirty-one kinases and 33 transcriptional factors were cold-responsive at protein, phosphopeptides, or site-specific phosphorylation levels. Our results showed that maize seedlings respond to cold shock rapidly, at both the proteome and phosphoproteome levels. This study provides a comprehensive landscape at the cold-responsive proteome and phosphoproteome in maize seedlings that can be a significant resource to understand how C4 plants respond to a sudden temperature drop.
HostingRepository	iProX
AnnounceDate	2022-06-01
AnnouncementXML	Submission_2022-09-28_23:49:22.746.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	zhiping Deng
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	iodoacetamide derivatized residue; phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-01 18:36:42	ID requested
1	2022-06-01 18:36:57	announced
⏵ 2	2022-09-28 23:49:23	announced	2022-09-29: Update publication information.

Xing J, Tan J, Feng H, Zhou Z, Deng M, Luo H, Deng Z, Integrative Proteome and Phosphoproteome Profiling of Early Cold Response in Maize Seedlings. Int J Mol Sci, 23(12):(2022) [pubmed]

submitter keyword: maize, cold stress, TMT-labeling, proteome, phosphoproteome, seedlings, photosynthesis, spliceosome

Zhiping Deng
contact affiliation	Zhejiang Academy of Agricultural Sciences
contact email	zhipingdeng@126.com
lab head
zhiping Deng
contact affiliation	Zhejiang Academy of Agricultural Sciences
contact email	zhipingdeng@126.com
dataset submitter

iProX dataset URI