PXD034256

PXD034256 is an original dataset announced via ProteomeXchange.

Title	FASII antibiotic adaptation reprograms Staphylococcus aureus behavior
Description	Proving functionality in the host environment is a crucial step in antimicrobial development pipelines. Antibiotics targeting fatty acid synthesis (FASII) of the major pathogen Staphylococcus aureus actively inhibit FASII but do not prevent in vivo growth, as bacteria compensate the FASII block by using environmental fatty acids. We used proteomics and phosphoproteomics to elucidate S. aureus responses to anti-FASII in host-relevant conditions. S. aureus responded to anti-FASII treatment in serum by massive reprogramming. A striking inverse correlation was observed in anti-FASII-adapted S. aureus, between amounts of stress response proteins that increase, and virulence factors that decrease. These findings suggest that anti-FASII adapted cells might be better prepared for survival and less equipped to damage the host. Infection by anti-FASII-adapted versus non-treated S. aureus was challenged in the Galleria mellonella model. Time to mortality was longer in insects infected by anti-FASII-treated bacteria compared to those infected by non-treated S. aureus. However, bacterial counts in infected dead insects were comparable for both groups. These results support the hypothesis that higher stress response and lower virulence factor expression, as shown here in FASII-antibiotic-adapted bacteria, may set the stage for persistent infection
HostingRepository	PRIDE
AnnounceDate	2023-10-09
AnnouncementXML	Submission_2023-10-09_09:35:15.982.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	CélineHenry
SpeciesList	scientific name: Staphylococcus aureus; NCBI TaxID: 1280;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-01 11:21:03	ID requested
⏵ 1	2023-10-09 09:35:16	announced

Dataset with its publication pending

submitter keyword: FASII antibiotic adaptation reprograms Staphylococcus aureus behavior

AlexandraGruss
contact affiliation	Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350, Jouy-en-Josas, France.
contact email	alexandra.gruss@inrae.fr
lab head
CélineHenry
contact affiliation	INRAE
contact email	celine.henry@inrae.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034256
     1. Label: PRIDE project
     2. Name: FASII antibiotic adaptation reprograms Staphylococcus aureus behavior