PXD034245

PXD034245 is an original dataset announced via ProteomeXchange.

Title	Subzero hydrogen-deuterium exchange CE-MS
Description	We have performed in solution hydrogen exchange (HDX) on peptide standards and bovine hemoglobin with subsequent quenching, pepsin digestion, and cold capillary electrophoretic separation coupled with mass spectrometry (MS) detection for the benchmarking of a lab built HDX-MS platform. Though neutral coated capillaries that eliminate electroosmotic flow provide fast separations with peak capaci-ties surpassing 200, uncoated capillaries with high electroosmotic flow achieve half as much peak ca-pacity with even faster separations but 30% higher deuterium retention for angiotensin II peptide standard. The data obtained from two different separation conditions on peptic digests of bovine he-moglobin shows strong agreement for relative deuterium uptake between methods and provides data amenable to software such as MS-Studio. Processed data from denatured hemoglobin vs the longest time point in this study (50,000s) also shows agreement with subunit interaction sites determined by crystallographic methods.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:56:50.151.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD034245
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Erik Jansson
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	monohydroxylated residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2022-06-01 05:01:48	ID requested
1	2022-12-22 11:43:53	announced
⏵ 2	2023-11-14 08:56:50	announced	2023-11-14: Updated project metadata.

10.6019/PXD034245;

submitter keyword: CE-MS, HDX, hemoglobin

Erik Jansson
contact affiliation	Department of Pharmaceutical Biosciences, Uppsala University
contact email	erik.jansson@farmbio.uu.se
lab head
Erik Jansson
contact affiliation	Uppsala University
contact email	erik.jansson@farmbio.uu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD034245
     1. Label: PRIDE project
     2. Name: Subzero hydrogen-deuterium exchange CE-MS