PXD034237 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of DVL3 polyglutamylation using Q-TOF |
Description | Polyglutamylation is a reversible post-translational modification initially discovered on tubulin. In eukaryotes, polyglutamylation is catalyzed by enzymes from the tubulin tyrosine ligase-like (TTLL) family, which show substrate and reaction specificities that are so far mostly explored on tubulin. By contrast, little is known on the mechanism and significance of TTLL-mediated modification of non-tubulin substrates. Here, we found that TTLL11 generates a previously unknown type of polyglutamylation by adding glutamate residues to the carboxy-terminus of a substrate protein. TTLL11 efficiently polyglutamylates the signaling protein disheveled 3 (DVL3), thereby changing the interactome of DVL3, as well as its capacity to undergo liquid-liquid phase separation (LLPS). Both carboxyterminal polyglutamylation and the resulting reduction in LLPS capacity of DVL3 were reverted by the deglutamylating enzyme CCP6, which demonstrates the causal relationship between TTLL11-mediated polyglutamylation and LLPS. We thus discovered a novel type of posttranslational modification catalyzed by a TTLL enzyme, which significantly broadens the range of proteins that can be modified by polyglutamylation, and provide first evidence that polyglutamylation can act as a regulator of protein LLPS. |
HostingRepository | PRIDE |
AnnounceDate | 2024-09-30 |
AnnouncementXML | Submission_2024-09-30_07:16:32.235.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD034237 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | David Potesil |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | impact II |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-06-01 03:53:37 | ID requested | |
⏵ 1 | 2024-09-30 07:16:32 | announced | |
Publication List
Keyword List
submitter keyword: TTLL11, WNT signaling, LC-MS, polyglutamylation,DVL3, interactome |
Contact List
Zbynek Zdrahal |
contact affiliation | Proteomics Core Facility and Research Group Proteomics, CEITEC-MU, Masaryk University, Brno, Czech Republic |
contact email | zdrahal@sci.muni.cz |
lab head | |
David Potesil |
contact affiliation | Proteomics Core Facility and Research Group Proteomics CEITEC-MU Masaryk University Brno, Czech Republic |
contact email | david.potesil@ceitec.muni.cz |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034237
- Label: PRIDE project
- Name: Analysis of DVL3 polyglutamylation using Q-TOF