PXD034218

PXD034218 is an original dataset announced via ProteomeXchange.

Title	Proteomics changes associated with the development of açaí (Euterpe oleracea Mart.) seeds
Description	In addition to being a source of polyphenols with beneficial health properties, açaí seeds are a rich source of mannans, which can be used to generate bioethanol or be converted to high-value D-mannose. Here we present a quantitative proteome dataset of açaí seeds at four stages of development (S1, S2, S3 and S4 stages), in which 2465 high confidence proteins were identified and 524 of them show statistically different abundance profiles during development. Several enzymes involved in the biosynthesis of nucleotide-sugars were quantified, especially those dedicated to the formation of GDP-mannose, which showed an increase in abundance between stages S1 and S3. Our data suggest that linear mannans present in high amounts in the cell walls of the endosperm of mature seeds are primarily deposited as galactomannans and during development they lose their galactosyl groups by alpha-galactosidase activity, which show increased abundances in the S3 and S4 stages. Additionally, we quantified the enzymes participating in the central pathway of flavonoid biosynthesis responsible for the formation of catechin and epicatechin, which are subunits of procyanidins, the main class of polyphenols in the açaí seeds.These proteins showed the same pattern of deposition, in which higher abundances were seen in the S1 and S2 stages. The dataset we offer now is a valuable starting point for a wide range of research aimed at exploiting the biotechnological potential of a troublesome by-product of the depulping process of açaí fruits.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:48:04.554.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Proteomics Unit
SpeciesList	scientific name: Euterpe oleracea; NCBI TaxID: 115466;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-30 11:42:55	ID requested
1	2024-07-01 21:21:49	announced
⏵ 2	2024-10-22 06:48:04	announced	2024-10-22: Updated project metadata.

10.1002/PMIC.202200251;

submitter keyword: plant proteomics, flavonoid biosynthesis, procyanidins, mannans,Euterpe oleracea

Fábio César Sousa Nogueira
contact affiliation	Federal University of Rio de Janeiro
contact email	fabiocsn@gmail.com
lab head
Proteomics Unit
contact affiliation	UFRJ
contact email	unidadeproteomica@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/07/PXD034218

PRIDE project URI

• PRIDE
  1. PXD034218
     1. Label: PRIDE project
     2. Name: Proteomics changes associated with the development of açaí (Euterpe oleracea Mart.) seeds