PXD034217

PXD034217 is an original dataset announced via ProteomeXchange.

Title	Establishment of TurboID proximity proteomics in the filamentous fungus Sordaria macrospora
Description	Biotin identification (BioID) is a proximity-dependent labeling technique to study protein-protein co-localization in vivo. Although BioID has been applied in animal cells, plants and yeast, the method remained to be established in filamentous fungi. In this study, we established BioID for the filamentous fungus Sordaria macrospora using the well-characterized striatin interacting phosphatase and kinase (STRIPAK) complex as a proof of principle. In detail, the STRIPAK complex interactor 1 (SCI1) was fused to a codon-optimized TurboID biotin ligase. This SCI1-TurboID fusion protein complemented the Δsci1 deletion strain phenotype. The identification of the already known SmSTRIPAK components PRO11, SmMOB3, SmPP2Ac1 and PRO22 in a BioID experiment with SCI1-TurboID demonstrated its successful application in S. macrospora. The technique will provide a powerful proteomics tool for fungal biologists.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:25:47.181.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Sordaria macrospora; NCBI TaxID: 5147;
ModificationList	biotinylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2022-05-30 11:42:37	ID requested
1	2022-10-25 09:11:40	announced
⏵ 2	2023-11-14 08:25:48	announced	2023-11-14: Updated project metadata.

Hollstein LS, Schmitt K, Valerius O, Stahlhut G, P, ö, ggeler S, Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora. Sci Rep, 12(1):17727(2022) [pubmed]

submitter keyword: Sordaria macrospora, TurboID, STRIPAK, proximity labeling, BioID

Stefanie Pöggeler
contact affiliation	Georg-August University, Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms
contact email	Stefanie.Poeggeler@biologie.uni-goettingen.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/10/PXD034217

PRIDE project URI

• PRIDE
  1. PXD034217
     1. Label: PRIDE project
     2. Name: Establishment of TurboID proximity proteomics in the filamentous fungus Sordaria macrospora