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PXD034217

PXD034217 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEstablishment of TurboID proximity proteomics in the filamentous fungus Sordaria macrospora
DescriptionBiotin identification (BioID) is a proximity-dependent labeling technique to study protein-protein co-localization in vivo. Although BioID has been applied in animal cells, plants and yeast, the method remained to be established in filamentous fungi. In this study, we established BioID for the filamentous fungus Sordaria macrospora using the well-characterized striatin interacting phosphatase and kinase (STRIPAK) complex as a proof of principle. In detail, the STRIPAK complex interactor 1 (SCI1) was fused to a codon-optimized TurboID biotin ligase. This SCI1-TurboID fusion protein complemented the Δsci1 deletion strain phenotype. The identification of the already known SmSTRIPAK components PRO11, SmMOB3, SmPP2Ac1 and PRO22 in a BioID experiment with SCI1-TurboID demonstrated its successful application in S. macrospora. The technique will provide a powerful proteomics tool for fungal biologists.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:25:47.181.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterOliver Valerius
SpeciesList scientific name: Sordaria macrospora; NCBI TaxID: 5147;
ModificationListbiotinylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF
Dataset History
RevisionDatetimeStatusChangeLog Entry
02022-05-30 11:42:37ID requested
12022-10-25 09:11:40announced
22023-11-14 08:25:48announced2023-11-14: Updated project metadata.
Publication List
Hollstein LS, Schmitt K, Valerius O, Stahlhut G, P, ö, ggeler S, Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora. Sci Rep, 12(1):17727(2022) [pubmed]
Keyword List
submitter keyword: Sordaria macrospora, TurboID, STRIPAK, proximity labeling, BioID
Contact List
Stefanie Pöggeler
contact affiliationGeorg-August University, Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms
contact emailStefanie.Poeggeler@biologie.uni-goettingen.de
lab head
Oliver Valerius
contact affiliationGeorg-August-Universität Göttingen
contact emailovaleri@gwdg.de
dataset submitter
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Dataset FTP location
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