PXD034217 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Establishment of TurboID proximity proteomics in the filamentous fungus Sordaria macrospora |
Description | Biotin identification (BioID) is a proximity-dependent labeling technique to study protein-protein co-localization in vivo. Although BioID has been applied in animal cells, plants and yeast, the method remained to be established in filamentous fungi. In this study, we established BioID for the filamentous fungus Sordaria macrospora using the well-characterized striatin interacting phosphatase and kinase (STRIPAK) complex as a proof of principle. In detail, the STRIPAK complex interactor 1 (SCI1) was fused to a codon-optimized TurboID biotin ligase. This SCI1-TurboID fusion protein complemented the Δsci1 deletion strain phenotype. The identification of the already known SmSTRIPAK components PRO11, SmMOB3, SmPP2Ac1 and PRO22 in a BioID experiment with SCI1-TurboID demonstrated its successful application in S. macrospora. The technique will provide a powerful proteomics tool for fungal biologists. |
HostingRepository | PRIDE |
AnnounceDate | 2023-11-14 |
AnnouncementXML | Submission_2023-11-14_08:25:47.181.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Oliver Valerius |
SpeciesList | scientific name: Sordaria macrospora; NCBI TaxID: 5147; |
ModificationList | biotinylated residue; phosphorylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2022-05-30 11:42:37 | ID requested | |
1 | 2022-10-25 09:11:40 | announced | |
⏵ 2 | 2023-11-14 08:25:48 | announced | 2023-11-14: Updated project metadata. |
Publication List
Hollstein LS, Schmitt K, Valerius O, Stahlhut G, P, ö, ggeler S, Establishment of in vivo proximity labeling with biotin using TurboID in the filamentous fungus Sordaria macrospora. Sci Rep, 12(1):17727(2022) [pubmed] |
Keyword List
submitter keyword: Sordaria macrospora, TurboID, STRIPAK, proximity labeling, BioID |
Contact List
Stefanie Pöggeler |
contact affiliation | Georg-August University, Institute of Microbiology and Genetics, Department of Genetics of Eukaryotic Microorganisms |
contact email | Stefanie.Poeggeler@biologie.uni-goettingen.de |
lab head | |
Oliver Valerius |
contact affiliation | Georg-August-Universität Göttingen |
contact email | ovaleri@gwdg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD034217
- Label: PRIDE project
- Name: Establishment of TurboID proximity proteomics in the filamentous fungus Sordaria macrospora